Determination of Organic Acids in Sheng-mai Injection by Ion Exclusion Chromatography

被引：3

作者：

Liu Rui ^{[1
,2
]}

Nie Li-Xing ^{[2
]}

Sun Huan ^{[3
]}

Li Tao ^{[3
]}

Wang Gang-Li ^{[2
]}

Lin Rui-Chao ^{[1
,2
]}

机构：

[1] Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100029, Peoples R China

[2] Natl Inst Food & Drug Control, Beijing 100050, Peoples R China

[3] Metrohm China Ltd, Cent Lab, Beijing 100005, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2012年 / 40卷 / 03期

关键词：

Ion exclusion chromatography; Organic acids; Sheng-mai injection;

D O I：

10.3724/SP.J.1096.2012.10799

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method was developed for the separation and determination of three organic acid, viz citric acid, malic acid and succinic acid in Sheng-mai Injection by ion exclusion chromatography with suppressed conductivity detection. The chromatographic separation was performed on a Transgenomic Sep-ION-300 column (300 mm length x 7.8 mm i.d., 8 mu m) at 70 degrees C and eluted with a mixture of 5 mmol/L sulphuric acid and acetone (95:5) at the flow rate of 0.4 mL/min. The effects of the concentration of sulphuric acid, the volume ratio of a cetone and column temperature were investigated and optimized. The calibration curve for citric acid, malic acid and succinic acid was in a good linearity in the range of 7.45-476.5 mg/L, 14-521.0 mg/L and 3.47-222.0 mg/L, respectively. The average recovery for citric acid, malic acid and succinic acid was 97.12% with RSD 2.7%, 96.9% with RSD 2.3%, and 95.09% with RSD 1.9%, respectively. The validated method was successfully applied to determination of organic acids in 15 batches of Sheng-mai Injection.

引用

页码：489 / 490

页数：2

共 5 条

[1] Jin GW, 2006, CHINESE J ANAL CHEM, V34, P987
[2] LIANG Zhi-De, 2001, J ANAL SCI, V17, P483
[3] Lin HY, 2007, CHIN J CHROMATOGR, V25, P107
[4] Ren Q, 2002, CHINESE J ANAL CHEM, V30, P304
[5] [杨元娟 YANG Yuan-Juan], 2009, [激光杂志, Laser Journal], V30, P94

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