Induction of rat facial nerve regeneration by functional collagen scaffolds

被引：65

作者：

Cao, Jiani ^{[1
,2
]}

Xiao, Zhifeng ^{[1
]}

Jin, Wei ^{[3
]}

Chen, Bing ^{[1
]}

Meng, Danqing ^{[1
]}

Ding, Wenyong ^{[1
]}

Han, Sufang ^{[1
]}

Hou, Xiaoshan ^{[3
]}

Zhu, Tiansheng ^{[3
]}

Yuan, Baoyu ^{[3
]}

Wang, Jing ^{[3
]}

Liang, Weibang ^{[3
]}

Dai, Jianwu ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China

[3] Nanjing Univ, Sch Med, Affiliated Drum Tower Hosp, Dept Neurosurg, Nanjing 210008, Peoples R China

来源：

BIOMATERIALS | 2013年 / 34卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Neurotrophic factors; Targeting repair; Laminin; Collagen; Peripheral nerve injury; CILIARY NEUROTROPHIC FACTOR; RETINAL GANGLION-CELLS; PERIPHERAL-NERVE; SCIATIC-NERVE; IN-VITRO; CNTF; GROWTH; CONDUIT; REPAIR; BDNF;

D O I：

10.1016/j.biomaterials.2012.10.031

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Nerve conduit provides a promising strategy for nerve regeneration, and the proper microenvironment in the lumen could improve the regeneration. Our previous work had demonstrated that linear ordered collagen scaffold (LOCS) could effectively guide the oriented growth of axons. Laminin is known as an important nerve growth promoting factor and can facilitate the growth cone formation. In addition, ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) can effectively improve the nerve regeneration after nerve injuries. However, in practice, diffusion caused by the body fluids is the major obstacle in their applications. To retain CNTF or BDNF on the scaffolds, we produced collagen binding CNTF (CBD-CNTF), collagen binding BDNF (CBD-BDNF) and laminin binding CNTF (LBD-CNTF), laminin binding BDNF (LBD-BDNF) respectively. In this work, we developed laminin modified LOCS fibers (L x LOCS) by chemical cross-linking LOCS fibers with laminin. Collagen binding or laminin binding neurotrophic factors were combined with LOCS or L x LOCS, and then filled them into the collagen nerve conduit They were found to guide the ordered growth of axons, and improve the nerve functional recovery in the rat facial nerve transection model. The combination of CNTF and BDNF greatly enhanced the facial nerve regeneration and functional recovery. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1302 / 1310

页数：9

共 46 条

[31]

Payne S H Jr, 2001, J South Orthop Assoc, V10, P173

[32] Nerve conduits and growth factor delivery in peripheral nerve repair [J].