Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation

被引:119
作者
Dou, Geng [1 ,2 ,3 ]
Tian, Ran [4 ]
Liu, Xuemei [1 ,2 ,3 ,5 ]
Yuan, Pingyun [4 ]
Ye, Qianwen [1 ,2 ,3 ]
Liu, Jin [1 ,2 ,3 ]
Liu, Siying [1 ,2 ,3 ]
Zhou, Jun [1 ,2 ,3 ]
Deng, Zhihong [1 ,2 ,3 ]
Chen, Xin [4 ]
Liu, Shiyu [1 ,2 ,3 ]
Jin, Yan [1 ,2 ,3 ]
机构
[1] Fourth Mil Med Univ, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
[2] Fourth Mil Med Univ, Sch Stomatol, Natl Clin Res Ctr Oral Dis, Xian 710032, Shaanxi, Peoples R China
[3] Fourth Mil Med Univ, Sch Stomatol, Ctr Tissue Engn, Shaanxi Key Lab Oral Dis, Xian 710032, Shaanxi, Peoples R China
[4] Xi An Jiao Tong Univ, Sch Chem Engn & Technol, Inst Polymer Sci Chem Engn, Dept Chem Engn,Shaanxi Key Lab Energy Chem Proc I, Xian 710049, Shaanxi, Peoples R China
[5] China Med Univ, Sch Stomatol, Dept Paediat Dent, Shenyang 110002, Liaoning, Peoples R China
基金
中国国家自然科学基金;
关键词
DRUG-DELIVERY; MACROPHAGE POLARIZATION; EXOSOMES; NANOPARTICLES; VEHICLES; CELLS; MECHANISMS; VESICLES; CURCUMIN;
D O I
10.1126/sciadv.aba2987
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Engineered extracellular vesicles (EVs) carrying therapeutic molecules are promising candidates for disease therapies. Yet, engineering EVs with optimal functions is a challenge that requires careful selection of functionally specific vesicles and a proper engineering strategy. Here, we constructed chimeric apoptotic bodies (cABs) for on-demand inflammation modulation by combining pure membrane from apoptotic bodies (ABs) as a bioconjugation/regulation module and mesoporous silica nanoparticles (MSNs) as a carrier module. MSNs were preloaded with anti-inflammatory agents (microRNA-21 or curcumin) and modified with stimuli-responsive molecules to achieve accurate cargo release at designated locations. The resulting cABs actively target macrophages in the inflammatory region and effectively promote M2 polarization of these macrophages to modulate inflammation due to the synergistic regulatory effects of AB membranes and the intracellular release of preloaded cargos. This work provides strategies to arbitrarily engineer modular EVs that integrate the advantages of natural EVs and synthetic materials for various applications.
引用
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页数:19
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