Studies on external electric field effects on absorption and fluorescence spectra of NADH

被引：13

作者：

Nakabayashi, Takakazu ^{[1
]}

Islam, Md. Serajul ^{[1
]}

Li, Liming ^{[2
]}

Yasuda, Masahide ^{[3
]}

Ohta, Nobuhiro ^{[1
]}

机构：

[1] Hokkaido Univ, RIES, Sapporo, Hokkaido 0010020, Japan

[2] Chitose Inst Sci & Technol, Dept Bio & Mat Photon, Chitose 0668655, Japan

[3] Miyazaki Univ, Dept Appl Chem, Fac Engn, Gakuen Kibanadai, Miyazaki 8892192, Japan

来源：

CHEMICAL PHYSICS LETTERS | 2014年 / 595卷

关键词：

NICOTINAMIDE ADENINE-DINUCLEOTIDE; CELLULAR-METABOLISM; INTRACELLULAR NADH; IMAGING REVEALS; LIFETIME; MICROSCOPY; DYNAMICS; STATE; AUTOFLUORESCENCE; DEPENDENCE;

D O I：

10.1016/j.cplett.2014.01.035

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Electric field effects on absorption and fluorescence spectra have been investigated for NADH that is a representative autofluorescent chromophore in cells. The change in electric dipole moment following absorption is significant in the electroabsorption spectrum, indicating charge transfer character in the excited state. The fluorescence intensity decreases in the presence of an electric field, which arises from the field-induced increase in the rate of the non-radiative process. The blue shift of the fluorescence spectrum and the increase in the fluorescence lifetime of NADH are measured in yeast cells, which is discussed in terms of a local electric field around NADH. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：25 / 30

页数：6

共 31 条

[1] Photophysical properties of a series of 4-aryl substituted 1,4-dihydropyridines [J].

Affeldt, Ricardo Ferreira ;

Iglesias, Rodrigo Sebastian ;

Rodembusch, Fabiano Severo ;

Russowsky, Dennis .

JOURNAL OF PHYSICAL ORGANIC CHEMISTRY, 2012, 25 (09) :769-777

[2] DYNAMIC STOKES SHIFT IN COUMARIN - IS IT ONLY RELAXATION [J].

AGMON, N .

JOURNAL OF PHYSICAL CHEMISTRY, 1990, 94 (07) :2959-2963

[3] Fluorescence Lifetime Measurements and Biological Imaging [J].

Berezin, Mikhail Y. ;

Achilefu, Samuel .

CHEMICAL REVIEWS, 2010, 110 (05) :2641-2684

[4] Distribution of mitochondrial NADH fluorescence lifetimes: Steady-state kinetics of matrix NADH interactions [J].

Blinova, K ;

Carroll, S ;

Bose, S ;

Smirnov, AV ;

Harvey, JJ ;

Knutson, JR ;

Balaban, RS .

BIOCHEMISTRY, 2005, 44 (07) :2585-2594

[5] Stark Realities [J].

Boxer, Steven G. .

JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (10) :2972-2983

[6] PULSE FLUORIMETRY STUDY OF OCTOPINE DEHYDROGENASE-REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE COMPLEXES [J].

BROCHON, JC ;

WAHL, P ;

MONNEUSEDOUBLET, MO ;

OLOMUCKI, A .

BIOCHEMISTRY, 1977, 16 (21) :4594-4599

[7] Tryptophan fluorescence shifts in proteins from hybrid simulations: An electrostatic approach [J].

Callis, PR ;

Burgess, BK .

JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (46) :9429-9432

[8] Multi-wavelength fluorescence lifetime spectroscopy: a new approach to the study of endogenous fluorescence in living cells and tissues [J].

Chorvat, D., Jr. ;

Chorvatova, A. .

LASER PHYSICS LETTERS, 2009, 6 (03) :175-193

[9] 1ST USE OF THE UV SUPER-ACO FREE-ELECTRON LASER - FLUORESCENCE DECAYS AND ROTATIONAL-DYNAMICS OF THE NADH COENZYME [J].

COUPRIE, ME ;

MEROLA, F ;

TAUC, P ;

GARZELLA, D ;

DELBOULBE, A ;

HARA, T ;

BILLARDON, M .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1994, 65 (05) :1485-1495

[10]

DIMROTH K, 1966, Z ANAL CHEM FRESENIU, V215, P344

← 1 2 3 4 →