Advanced oxidation protein products enhances soluble Fms-like tyrosine kinase 1 expression in trophoblasts: A possible link between oxidative stress and preeclampsia

被引：39
作者：
Huang, Q. T. [1 ]
Wang, S. S. [1 ]
Zhang, M. [1 ]
Huang, L. P. [1 ]
Tian, J. W. [2 ]
Yu, Y. H. [1 ]
Wang, Z. J. [1 ]
Zhong, M. [1 ]
机构：
[1] Southern Med Univ, Nanfang Hosp, Div Obstet & Gynecol, Guangzhou 510515, Guangdong, Peoples R China
[2] Southern Med Univ, Nanfang Hosp, Nephrol Res Ctr Guangdong Prov, Div Nephrol,Key Lab Organ Failure Res, Guangzhou 510515, Guangdong, Peoples R China
来源：
PLACENTA | 2013年 / 34卷 / 10期
关键词：
Advanced oxidation protein products; Trophoblast; Soluble Fms-like tyrosine kinase 1; CIRCULATING ANGIOGENIC FACTORS; INSULIN-RESISTANCE; CELLS; WOMEN; INFLAMMATION; MECHANISMS; PREGNANCY; HYPOXIA; SFLT-1; RISK;
D O I：
10.1016/j.placenta.2013.06.308
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Accumulation of advanced oxidation protein products (AOPPs) is prevalent in obesity, advanced maternal age, diabetes mellitus, and polycystic ovary syndrome. Alterations in the regulation and signaling of angiogenic pathways have been recognized as a link between these conditions and pre-eclampsia. To investigate the possible impact of AOPPs on soluble Fms-like tyrosine kinase 1 (sFlt-1) expression in trophoblasts. A trophoblast cell line (HRT-8/SVneo) was treated with various concentrations of AOPPs. The mRNA expression of sFlt-1, vascular endothelial growth factor (VEGF), and placental growth factor (PIGF) in trophoblasts were measured with the use of real-time polymerase chain reaction; and the secretion of sFlt-1, VEGF, and PIGF protein from trophoblasts were detected with the use of ELISA. Exposure of HRT-8/SVneo cells to AOPPs induced overexpression of sFlt-1 at mRNA and protein levels in a dose dependent manner. These effects could be inhibited by apocynin, an inhibitors of NADPH oxidase. Our data identified AOPPs as a class of important mediator in the regulation and signaling of angiogenic pathways of trophoblasts. Accumulation of AOPPs might contributes to the pathogenesis of preeclampsia by promoting sFlt-1 production in trophoblasts. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：949 / 952
页数：4
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