Isolation and characterization of a mutant of Saccharomyces cerevisiae affected in the FLO1 locus

被引：7

作者：

Reboredo, NM ^{[1
]}

Sieiro, C ^{[1
]}

Blanco, P ^{[1
]}

Villa, TG ^{[1
]}

机构：

[1] UNIV SANTIAGO DE COMPOSTELA,FAC FARM,DEPT MICROBIOL,E-15706 SANTIAGO,SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1996年 / 137卷 / 01期

关键词：

Saccharomyces cerevisiae; flocculation mutant;

D O I：

10.1016/0378-1097(96)00032-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A non-flocculent strain of Saccharomyces cerevisiae was selected after EMS mutation of a flocculent and heterozygous FLOl locus diploid. The analysis of 25 asci from this diploid showed in all cases segregation 0F:4NF, thus confirming that it was probably affected in the desired gene. After sporulation and dissection of asci, three haploid strains were chosen, which were altered in the locus FLOl. Crossing these three strains with two other ones having markers for ADEl and pholl::LEU2, we could map the mutation at ca. 4.3 cM and ca. 37.7 cM from the PHOll and ADEl loci respectively.

引用

页码：57 / 61

页数：5

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