Binding of cytoskeletal proteins with silver nanoparticles

被引：34

作者：

Wen, Yimei ^{[1
]}

Geitner, Nicholas K. ^{[1
]}

Chen, Ran ^{[1
]}

Ding, Feng ^{[2
]}

Chen, Pengyu ^{[3
]}

Andorfer, Rachel E. ^{[1
]}

Govindan, Praveen Nedumpully ^{[2
]}

Ke, Pu Chun ^{[1
]}

机构：

[1] Clemson Univ, Nanobiophys & Soft Matter Lab, Clemson, SC 29634 USA

[2] Clemson Univ, Struct Dynam & Funct Biomol & Mol Complexes Lab, Clemson, SC 29634 USA

[3] Univ Michigan Ann Arbor, Microsyst Technol & Sci Lab, Ann Arbor, MI 48109 USA

来源：

RSC ADVANCES | 2013年 / 3卷 / 44期

基金：

美国国家科学基金会;

关键词：

CORONA; TOXICITY; SIZE; MICROTUBULES; TRANSFERRIN; ACTIVATION; DYNAMICS; RELEASE; IONS;

D O I：

10.1039/c3ra43281e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have characterized the binding of cytoskeletal proteins, namely, tubulin and actin, with silver nanoparticles using the techniques of dynamic light scattering, UV-Vis spectrophotometry, circular dichroism spectroscopy, hyperspectral imaging, and transmission electron microscopy. Overall, actin displayed a higher propensity than tubulin for silver nanoparticles while both proteins experienced conformational changes upon binding. Conversely, ion release from silver nanoparticles was significantly compromised upon the formation of protein biocoronas, as shown by inductively coupled plasma mass spectroscopy. The implications of cytoskeletal protein biocorona on the transformation and cytotoxicity of silver nanoparticles have been discussed.

引用

页码：22002 / 22007

页数：6

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