Are morphological criteria sufficient for the identification of circulating tumor cells in renal cancer?

被引：46

作者：

El-Heliebi, Amin ^{[1
]}

Kroneis, Thomas ^{[1
]}

Zoehrer, Evelyn ^{[1
]}

Haybaeck, Johannes ^{[2
]}

Fischereder, Katja ^{[3
]}

Kampel-Kettner, Karin ^{[3
]}

Zigeuner, Richard ^{[3
]}

Pock, Hannelore ^{[4
]}

Riedl, Regina ^{[5
]}

Stauber, Rudolf ^{[4
]}

Geigl, Jochen Bernd ^{[6
]}

Huppertz, Berthold ^{[1
]}

Sedlmayr, Peter ^{[1
]}

Lackner, Carolin ^{[2
]}

机构：

[1] Med Univ Graz, Inst Cell Biol Histol & Embryol, A-8010 Graz, Austria

[2] Med Univ Graz, Inst Pathol, A-8036 Graz, Austria

[3] Med Univ Graz, Dept Urol, A-8036 Graz, Austria

[4] Med Univ Graz, Dept Internal Med, Div Gastroenterol & Hepatol, A-8036 Graz, Austria

[5] Med Univ Graz, Inst Med Informat Stat & Documentat, A-8036 Graz, Austria

[6] Med Univ Graz, Inst Human Genet, A-8010 Graz, Austria

来源：

JOURNAL OF TRANSLATIONAL MEDICINE | 2013年 / 11卷

关键词：

Circulating tumor cells; Circulating tumor microemboli; Renal cancer; ScreenCell (R); Array comparative genomic hybridization (array-CGH); Circulating endothelial cells; COMPARATIVE GENOMIC HYBRIDIZATION; ENDOTHELIAL-CELLS; SINGLE CELLS; PERIPHERAL-BLOOD; BREAST-CANCER; ARRAY-CGH; CARCINOMA; AMPLIFICATION; SIZE; EVOLUTION;

D O I：

10.1186/1479-5876-11-214

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background: Single circulating tumor cells (CTCs) or circulating tumor microemboli (CTMs) are potential biomarkers of renal cell cancer (RCC), however studies of CTCs/CTMs in RCC are limited. In this pilot study we aimed to evaluate a novel blood filtration technique suited for cytomorphological classification, immunocytochemical and molecular characterization of filtered, so called circulating non-hematologic cells (CNHCs) - putative CTCs/CTMs - in patients with RCC. Methods: Blood of 40 patients with renal tumors was subjected to ScreenCell (R) filtration. CNHCs were classified according to cytomorphological criteria. Immunocytochemical analysis was performed with antibodies against CD45, CD31 and carbonic anhydrase IX (CAIX, a RCC marker). DNA of selected CNHCs and respective primary tumors was analysed by array-CGH. Results: CNHC-clusters with malignant or uncertain malignant cytomorphological features - putative CTMs - were negative for CD45, positive for CD31, while only 6% were CAIX positive. Array-CGH revealed that 83% of malignant and uncertain malignant cells did represent with a balanced genome whereas 17% presented genomic DNA imbalances which did not match the aberrations of the primary tumors. Putative single CTCs were negative for CD45, 33% were positive for CD31 and 56% were positive for CAIX. Conclusions: The majority of CNHC-clusters, putative CTMs, retrieved by ScreenCell (R) filtration may be of endothelial origin. Morphological criteria seem to be insufficient to distinguish malignant from non-malignant cells in renal cancer.

引用

页数：17

共 70 条

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