An improved method for seed-bank analysis: Seedling emergence after removing the soil by sieving

被引:1
作者
TerHeerdt, GNJ [1 ]
Verweij, GL [1 ]
Bekker, RM [1 ]
Bakker, JP [1 ]
机构
[1] MINIST TRANSPORT & PUBL WORKS,DIRECTORATE IJSSELMEEGEBIED,8200 AP LELYSTAD,NETHERLANDS
关键词
sample size; seed distribution; seed separation method; seedling emergence method; seedling emergence rates;
D O I
暂无
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
1. The seedling emergence method for assessing the size of the seed bank is improved by washing soil samples on a fine sieve and spreading the thus concentrated samples in a 3-5 mm thick layer on sterilized potting compost. 2. The method largely increases the number of seedlings that emerge as compared to unconcentrated samples. Hand-sorting afterwards shows that the germination rates vary between 81 and 100% of the viable seeds present. 3. Ninety-five per cent of the seedlings will emerge within 6 weeks using this method. 4. The method greatly reduces the greenhouse space needed and enables examination of large sample volumes.
引用
收藏
页码:144 / 151
页数:8
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