Glutamine Protects Intestinal Barrier Function of Colon Epithelial Cells from Ethanol by Modulating Hsp70 Expression

被引：22

作者：

Akagi, Reiko ^{[1
]}

Ohno, Michiko ^{[3
]}

Matsubara, Kiminori ^{[2
]}

Fujimoto, Mitsuaki ^{[4
]}

Nakai, Akira ^{[4
]}

Inouye, Sachiye ^{[1
]}

机构：

[1] Yasuda Womens Univ, Fac Pharm, Dept Pharm, Hiroshima, Japan

[2] Hiroshima Univ, Grad Sch Educ, Dept Human Life Sci Educ, Hiroshima 7310153, Japan

[3] Nara Womens Univ, Fac Human Life & Environm, Dept Food Sci & Nutr, Nara 630, Japan

[4] Yamaguchi Univ, Dept Biochem & Mol Biol, Sch Med, Yamaguchi, Japan

来源：

PHARMACOLOGY | 2013年 / 91卷 / 1-2期

关键词：

Heat shock protein 70; Heat shock factor 1; Glutamine; Ethanol; Barrier function; HEAT-SHOCK PROTEINS; MOLECULAR CHAPERONES; IMPROVES SURVIVAL; PERMEABILITY; CACO-2; INJURY; HYPERTHERMIA; INCREASE; BINDING;

D O I：

10.1159/000345930

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In this study, we investigated the protective effect of glutamine on barrier dysfunction induced by ethanol, by using human epithelial colorectal adenocarcinoma cells (Caco-2). Our results show that addition of glutamine to culture medium significantly improved the disruption of integrity caused by ethanol, which was associated with increased expression of heat shock protein 70 (Hsp70). Ethanol exposure moderately activates heat shock factor 1 (HSF1), which was characterized by increased DNA-binding activity and phosphorylation status of HSF1. Remarkably, glutamine treatment enhanced ethanol-mediated expression of Hsp70 and activation of HSF1. Up-regulation of Hsp70 by pretreatment with heat stress also promoted recovery from the ethanol-induced barrier dysfunction. Taken together, these observations indicate that glutamine protects the intestinal barrier function in Caco-2 cells, in part by modulating HSF1-mediated Hsp70 expression. Copyright (c) 2013 S. Karger AG, Basel

引用

页码：104 / 111

页数：8

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