Bovine tuberculosis in India: Potential basis for zoonosis

被引：55

作者：

Prasad, HK ^{[1
]}

Singhal, A

Mishra, A

Shah, NP

Katoch, VM

Thakral, SS

Singh, DV

Chumber, S

Bal, S

Aggarwal, S

Padma, MV

Kumar, S

Singh, MK

Acharya, SK

机构：

[1] All India Inst Med Sci, Dept Biotechnol, New Delhi 110029, India

[2] Cent JALMA Inst Leprosy & Mycobacterial Dis, Agra 282001, Uttar Pradesh, India

[3] Cent Mil Vet Lab, Meerut 250001, Uttar Pradesh, India

[4] All India Inst Med Sci, Dept Surg, New Delhi 110029, India

[5] All India Inst Med Sci, Dept Paediat Surg, New Delhi 110029, India

[6] All India Inst Med Sci, Dept Neurol, New Delhi 110029, India

[7] All India Inst Med Sci, Dept Obstet & Gynecol, New Delhi 110029, India

[8] All India Inst Med Sci, Dept Pathol, New Delhi 110029, India

[9] All India Inst Med Sci, Dept Gastroenterol, New Delhi 110029, India

来源：

TUBERCULOSIS | 2005年 / 85卷 / 5-6期

关键词：

zoonotic TB; M; tuberculosis; bovis; nested PCR; HupB;

D O I：

10.1016/j.tube.2005.08.005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Our laboratory has designed a specific nested-PCR (N-PCR) assay, based on the hupB gene of Mycobacterium tuberculosis (Rv2986c) and Mycobacterium bovis (Mb3010c) as a method to differentiate these closely related species. The present paper deciphers the utility of this assay for identification of pathogenic Mycobacteria in clinical samples. Extra-pulmonary clinical samples obtained from cattle and humans were investigated. Pre-dominance of M. tuberculosis (15.7%) and M. bovis (26.8%) was seen in humans and cattle, respectively. However, more importantly, both mycobacterial pathogens (mixed infection) were identified in a number of samples. In humans 8.7% of the samples and 35.7% in cattle were classified as mixed infection. The detection of mixed infection with the mycoabcterial pathogenic duo in humans and bovines denotes the prospect of potential transmission of these pathogens from humans to cattle (zoonosis) and vice versa (reverse zoonosis). (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：421 / 428

页数：8

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