Prevalence and dissemination of the Ser315Thr substitution within the KatG enzyme in isoniazid-resistant strains of Mycobacterium tuberculosis isolated in Uruguay

被引：8
|
作者：
Varela, Gustavo [1 ]
Gonzalez, Sabina [1 ]
Gadea, Pilar [1 ]
Coitinho, Cecilia [2 ]
Mota, Ines [1 ]
Gonzalez, Gladys [1 ]
Goni, Fernando [3 ,4 ]
Rivas, Carlos [2 ]
Schelotto, Felipe [1 ]
机构：
[1] Univ Republica, Inst Hyg, Dept Bacteriol & Virol, Montevideo, Uruguay
[2] Honorary AntiTB Commiss, Dept Lab, Montevideo, Uruguay
[3] Univ Republica, Sch Chem, Dept Immunol, Montevideo, Uruguay
[4] NYU, Sch Med, Dept Neurol, New York, NY USA
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2008年 / 57卷 / 12期
关键词：
D O I：
10.1099/jmm.0.2008/001917-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The aim of this study was to determine the prevalence of Ser315Thr substitution in isoniazid (INH)-resistant strains of Mycobacterium tuberculosis in Uruguay. The katG gene of 62 INH-resistant strains was analysed by an RFLP-PCR assay. PCR products were digested with Mspl to detect Ser315Thr and Arg463Leu substitutions. A total of 16 of the 62 (26%) INH-resistant strains analysed had a Ser315Thr substitution. Only one INH-resistant strain had an Arg463Leu substitution and two strains had a deletion in katG. Of the 16 strains with Ser315Thr, 15 showed different profiles using a double-repetitive-element PCR assay, demonstrating that there was no local dissemination of any particular strain. These findings are in agreement with published data from regions where the prevalence of tuberculosis (TB) is intermediate and may be due in part to the success of the local TB control programme.
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页码：1518 / 1522
页数：5
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