Characterization of the Epicardial Adipose Tissue in Decellularized Human-Scaled Whole Hearts: Implications for the Whole-Heart Tissue Engineering

被引：5

作者：

Akhyari, Payam ^{[1
]}

Oberle, Fabian ^{[1
]}

Huelsmann, Joern ^{[1
]}

Heid, Hans ^{[2
]}

Lehr, Stefan ^{[3
]}

Barbian, Andreas ^{[4
]}

Nakanishi, Sentaro ^{[1
,5
]}

Aubin, Hug ^{[1
]}

Jenke, Alexander ^{[1
]}

Lichtenberg, Artur ^{[1
]}

机构：

[1] Heinrich Heine Univ, Fac Med, Dept Cardiovasc Surg, Res Grp Expt Surg, Moorenstr 5, D-40225 Dusseldorf, Germany

[2] German Canc Res Ctr, Helmholtz Grp Cell Biol, Heidelberg, Germany

[3] German Diabet Ctr, Prote Grp, Dusseldorf, Germany

[4] Heinrich Heine Univ, Fac Med, Div Clin Anat, Core Facil Electron Microscopy, Dusseldorf, Germany

[5] Asahikawa Med Univ, Dept Cardiac Surg, Asahikawa, Hokkaido, Japan

来源：

TISSUE ENGINEERING PART A | 2018年 / 24卷 / 7-8期

关键词：

whole-heart decellularization; epicardial adipose tissue; myocardial extracellular matrix; PERFUSION DECELLULARIZATION; MATRIX; TRANSPLANTATION; MYOCARDIUM;

D O I：

10.1089/ten.tea.2017.0107

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Whole-organ engineering is an innovative field of regenerative medicine with growing translational perspectives. Recent reports suggest the feasibility of decellularization and repopulation of entire human size hearts. However, little is known about the susceptibility of epicardial adipose tissue (EAT) to decellularization. In this study, human size hearts of ovine donors were subjected to perfusion-based decellularization using detergent solutions. Upon basic histological evaluation and total DNA measurement myocardial regions prove largely decellularized while EAT demonstrated cellular remnants, further confirmed by transmission electron microscopy. Western blot analysis showed a significant reduction in lipid-associated and cardiac proteins. However, gas chromatography revealed unchanged proportional composition of fatty acids in EAT of decellularized whole hearts. Finally, cell culture medium conditioned with EAT from decellularized whole hearts had a significant deleterious effect on cardiac fibroblasts. These data suggest that perfusion decellularization of human size whole hearts provides inconsistent efficacy regarding donor material removal from myocardial regions as opposed to EAT.

引用

页码：682 / 693

页数：12

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