ER regulates lipid metabolism in bone through ATGL and perilipin

被引:38
作者
Wend, Korinna [1 ,2 ,3 ]
Wend, Peter [4 ]
Drew, Brian G. [5 ]
Hevener, Andrea L. [5 ]
Miranda-Carboni, Gustavo A. [4 ]
Krum, Susan A. [1 ,2 ,3 ]
机构
[1] Univ Calif Los Angeles, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Orthopaed Surg, Orthopaed Hosp, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, David Geffen Sch Med, Res Ctr, Orthopaed Hosp, Los Angeles, CA 90095 USA
[4] Univ Calif Los Angeles, David Geffen Sch Med, Jonsson Comprehens Canc Ctr, Dept Obstet & Gynecol, Los Angeles, CA 90095 USA
[5] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Div Endocrinol Diabet & Hypertens, Los Angeles, CA 90095 USA
基金
美国国家卫生研究院;
关键词
ESTROGEN RECEPTOR; ESTROGEN; ADIPOCYTES; LIPID DROPLET; ESTROGEN-RECEPTOR-ALPHA; MARROW ADIPOCYTES; DIFFERENTIATION; DROPLETS; ADIPOGENESIS; OSTEOPOROSIS; OSTEOBLAST; RESORPTION; DELETION; OBESITY;
D O I
10.1002/jcb.24470
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A decrease in bone mineral density during menopause is accompanied by an increase in adipocytes in the bone marrow space. Ovariectomy also leads to accumulation of fat in the bone marrow. Herein we show increased lipid accumulation in bone marrow from estrogen receptor alpha (ER) knockout (ERKO) mice compared to wild-type (WT) mice or estrogen receptor beta (ER) knockout (ERKO) mice. Similarly, bone marrow cells from ERKO mice differentiated to adipocytes in culture also have increased lipid accumulation compared to cells from WT mice or ERKO mice. Analysis of individual adipocytes shows that WT mice have fewer, but larger, lipid droplets per cell than adipocytes from ERKO or ERKO animals. Furthermore, higher levels of adipose triglyceride lipase (ATGL) protein in WT adipocytes correlate with increased lipolysis and fewer lipid droplets per cell and treatment with 17-estradiol (E2) potentiates this response. In contrast, cells from ERKO mice display higher perilipin protein levels, promoting lipogenesis. Together these results demonstrate that E2 signals via ER to regulate lipid droplet size and total lipid accumulation in the bone marrow space in vivo. J. Cell. Biochem. 114: 13061314, 2013. (c) 2012 Wiley Periodicals, Inc.
引用
收藏
页码:1306 / 1314
页数:9
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