Spectrophotometric and spectrofluorimetric determination of pefloxacin

Two methods were adopted for the analysis of Pefloxacin. The first was a spectrophotometric determination of the substance in 0.1N H2SO4 measuring the absorbance at lambda 276 nm. Concentrations adhering to Beer's law were from 2.0-10.0 mu g ml(-1). The method gave satisfactory results when applied on Peflacine tablets after extracting the active substance completely with alcohol The validity of this method was ascertained by standard addition technique, the mean percentage recovery +/- S.D. was found to be [99.87 +/- 0.96]. The second was a spectrofluorimetric method in which samples of Pefloxacin in 0.1N H2SO4 showed native fluorecence at lambda 445 nm when excitation was at lambda 282 nm. The calibration graph was rectilinear from 0.2 - 1.2 mu g ml(-1). The method was applied to Peflacine tablets after complete extraction of Pefloxacin with alcohol and the results were satisfactory. The validity of the method was ascertained by the standard addition technique, the mean percentage recovery +/- S.D. was found to be [100.14 +/- 0.52]. The second method was ten times more sensitive than the first one. The proposed methods were sensitive,accurate and precise as compared with the HPLC method of analysis of Pefloxacin.