Analysis of proteins and peptides by electromigration methods in microchips

被引：46

作者：

Stepanova, Sille ^{[1
]}

Kasicka, Vaclav ^{[1
]}

机构：

[1] Czech Acad Sci, Inst Organ Chem & Biochem, Flemingovo 2, Prague 16610 6, Czech Republic

来源：

JOURNAL OF SEPARATION SCIENCE | 2017年 / 40卷 / 01期

关键词：

Microchip electrophoresis; Microfluidics; Peptides; Proteins; ELECTROPHORESIS-ELECTROSPRAY-IONIZATION; MICROFLUIDIC CHIP ELECTROPHORESIS; ISOELECTRIC POINT DETERMINATION; CAPILLARY GEL-ELECTROPHORESIS; PHOTOIMMOBILIZED PH GRADIENT; FREE-FLOW ELECTROPHORESIS; SOLID-PHASE EXTRACTION; CARDIAC TROPONIN-I; MASS-SPECTROMETRY; ON-CHIP;

D O I：

10.1002/jssc.201600962

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This review presents the developments and applications of microchip electromigration methods in the separation and analysis of peptides and proteins in the period 2011-mid-2016. The developments in sample preparation and preconcentration, microchannel material, and surface treatment are described. Separations by various microchip electromigration methods (zone electrophoresis in free and sieving media, affinity electrophoresis, isotachophoresis, isoelectric focusing, electrokinetic chromatography, and electrochromatography) are demonstrated. Advances in detection methods are reported and novel applications in the areas of proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices, and determination of physicochemical parameters are shown.

引用

页码：228 / 250

页数：23

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