Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays

被引：7

作者：

Avino, Anna ^{[1
,2
]}

Jose Gomara, Maria ^{[3
]}

Malakoutikhah, Morteza ^{[3
]}

Haro, Isabel ^{[3
]}

Eritja, Ramon ^{[1
,2
]}

机构：

[1] CIBER BBN Networking Ctr Bioengn Biomat & Nanomed, Inst Adv Chem Catalonia IQAC CSIC, E-08034 Barcelona, Spain

[2] Inst Res Biomed IRB Barcelona, E-08028 Barcelona, Spain

[3] Inst Adv Chem Catalonia IQAC CSIC, Unit Synth & Biomed Applicat Peptides, E-08034 Barcelona, Spain

来源：

MOLECULES | 2012年 / 17卷 / 12期

关键词：

oligonucleotide-peptide conjugates; ELISA; solid-phase synthesis; rheumatoid arthritis; epitopes; anti-citrullinated protein/peptide antibodies; ACPA; fibrin; filaggrin; citrullinated peptides; DNA-DIRECTED IMMOBILIZATION; ANTIBODIES; HYBRIDIZATION; ATTACHMENT; DELIVERY;

D O I：

10.3390/molecules171213825

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprotection allowed the preparation of polypyrimidine oligonucleotides carrying longer and arginine-rich peptides. An ELISA-based test using the oligonucleotide-citrullinated peptide conjugates was developed for the detection of anti-citrullinated protein/peptide antibodies in human serum from rheumatoid arthritis patients.

引用

页码：13825 / 13843

页数：19

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