Rapid detection of subtype-selective nuclear hormone receptor binding with bacterial genetic selection

被引：10

作者：

Skretas, G

Wood, DW

机构：

[1] Princeton Univ, Dept Chem Engn, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 12期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1128/AEM.71.12.8995-8997.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Subtype-selective nuclear hormone receptor modulators could potentially allow the development of valuable tissue-specific therapeutics. A simple biosensor that allows subtype-specific nuclear hormone receptor binding to be reflected by the growth phenotype of Escherichia coli cells has been constructed. This system will potentially enable the facile detection or evolution of subtype-selective hormone analogues.

引用

收藏

页码：8995 / 8997

页数：3

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