Lysobacter rhizophilus sp nov., isolated from rhizosphere soil of mugunghwa, the national flower of South Korea

A Gram-staining-negative, aerobic, motile, rod-shaped, catalase- and oxidase-negative strain with one polar flagellum, designated THG-YS3.6(T), was isolated from rhizosphere soil of a mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-37 degrees C (optimum 25-30 degrees C), at pH 6-8 (optimum 7.0) and with 0-2.0% NaCl (optimum 1 %). The isoprenoid quinone was ubiquinone-8 (Q-8). The major cellular fatty acids were iso-C-11 (: 0), iso-C-11 : 0 3-OH, iso-C-15 : 0, iso-C-16 : 0, C-16 (: 1)omega 7c alcohol, C-16 : 0, iso-C-17 : 0 and summed feature 9 (iso-C-17 : 1 omega 9c and/or C-16 : 0 10-methyl). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unknown phospholipid, one unknown lipid and three unknown aminolipids. The DNA G+C content of strain THG-YS3.6(T) was 65.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.6(T) were identified as Lysobacter yangpyeongensis KACC 11407(T) (98.7 %), Lysobacter oryzae KCTC 22249(T) (98.0 %), Lysobacter niabensis KACC 11587(T) (97.6 %) and Lysobacter terrae KACC 17646(T) (97.1 %). The DNA-DNA relatedness values between strain THG-YS3.6 T and L. yangpyeongensis KACC 11407(T), L. oryzae KCTC 22249(T), L. niabensis KACC 11587(T) and L. terrae KACC 17646(T) were 53.8 +/- 1.0 %, 12.9 +/- 1.2 %, 10.9 +/- 0.6% and 7.0 +/- 1.9 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.6(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter rhizophilus sp. nov. is proposed. The type strain is THG-YS3.6(T) (=KCTC 52082(T) =CCTCC AB 2015358(T)).