Antigenicity of poly(dA-dT)center dot poly(dA-dT) photocrosslinked with 8-methoxypsoralen

被引:18
作者
Arif, Z [1 ]
Ali, R [1 ]
机构
[1] ALIGARH MUSLIM UNIV,DEPT BIOCHEM,FAC MED,ALIGARH 202002,UTTAR PRADESH,INDIA
关键词
D O I
10.1006/abbi.1996.0208
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report the formation of photocrosslink (diadduct) between duplex poly(dA-dT) and 8-methoxy-psoralen inferred on the basis of UV and fluorescence characteristics, T-m, nuclease S1 digestibility, and hydroxyapatite column chromatography. The photo-crosslink was highly immunogenic inducing high titer antibodies in goat. The affinity constant of antigen-antibody interaction quantitated by precipitin titration was found to be 2.71 x 10(9) M(-1), calculated by Langmuir isotherm plot. These antibodies were highly specific showing recognition of almost exclusively of nucleic acid-furocoumarin photoadducts and could be used as a probe to detect and quantitate DNA modified with furocoumarins in health and disease. Antibodies against poly(dA-dT)-furocoumarin photocrosslink might be a better probe because of repeated A-T sequences on the immunogen. (C) 1996 Academic Press, Inc.
引用
收藏
页码:191 / 198
页数:8
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