A role for cell-surface CSF-1 in osteoblast-mediated osteoclastogenesis

被引:35
作者
Yao, GQ
Sun, BH
Weir, EC
Insogna, KL
机构
[1] Univ Edinburgh, Edinburgh, Midlothian, Scotland
[2] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06520 USA
关键词
cell surface; CSF-1; osteoclastogenesis; co-culture;
D O I
10.1007/s00223-001-1079-x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
CSF-1 is required for osteoblast-mediated osteoclast formation. Osteoblasts produce soluble (sCSF-1) and cell-surface forms of CSF-1 (also known as membrane-bound CSF-1, mCSF-1) but their individual contributions to osteoclastogenesis remain unclear. Using glutaraldehyde-fixed primary murine osteoblasts as a source of mCSF-1, osteoblasts from op/ op mice as a source for other osteoblast-derived osteoclastogenic factors and murine bone marrow as a source of osteoclast progenitors, osteoclast-like cells (OCL) formation was observed after 7-9 days of co-culture. In contrast, no OCL formation occurred when mCSF-1 expressed by primary murine osteoblasts was blocked by CSF-1 antibody pretreatment or when op/op osteoblasts were substituted for primary murine osteoblasts in the co-culture system. Osteoclast formation was also significantly inhibited when murine primary osteoblasts were pretreated with an antisense phosphorothioate oligonucleotide against mCSF-1. Finally, mCSF-1 and sCSF-1 were synergistic in stimulating OCL formation. These data support the conclusion that mCSF-1 plays an important role in osteoblast-mediated osteoclastogenesis within the bone microenvironment.
引用
收藏
页码:339 / 346
页数:8
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