Effects of dietary Bacillus subtilis C-3102 on the production, intestinal cytokine expression and autochthonous bacteria of hybrid tilapia Oreochromis niloticus ♀ x Oreochromis aureus ♂

被引：90

作者：

He, Suxu ^{[1
]}

Zhang, Yu ^{[1
]}

Xu, Li ^{[1
]}

Yang, Yalin ^{[1
]}

Marubashi, Toshihiro ^{[2
]}

Zhou, Zhigang ^{[1
]}

Yao, Bin ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100081, Peoples R China

[2] Calpis Co Ltd, Tokyo 1500021, Japan

来源：

AQUACULTURE | 2013年 / 412卷

基金：

中国国家自然科学基金;

关键词：

Bacillus subtilis; Cytokine expression; Autochthonous microbiota; Hybrid tilapia; TROUT ONCORHYNCHUS-MYKISS; GROWTH-PERFORMANCE; RAINBOW-TROUT; PROBIOTIC BACTERIA; IMMUNE-RESPONSES; DISEASE RESISTANCE; GENE-EXPRESSION; SURVIVAL; GROUPER; MICROBIOTA;

D O I：

10.1016/j.aquaculture.2013.06.028

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Bacillus spp. are widely used in aquafeeds as probiotics for enhanced growth performance, innate immune responses, and disease resistance. The present study aimed to evaluate the effects of low doses of dietary Bacillus subtilis C-3102 on the production, intestinal cytokine expression and adhesive bacteria of hybrid tilapia Oreochromis niloticus female x Oreochromis aureus male. Juvenile hybrid tilapia were fed B. subtilis C-3102 at doses of 0 (CK), 2.5 x 10(5) (Ta) and 5.0 x 10(5) (Tb) CFU g(-1) of diets for 56 days. The results showed that different diets had no effects on fish growth performance. The B. subtilis supplement was detected in the gut wall of fish treated with 5.0 x 10(5) CFU g(-1) at 28 days and 56 days by both plate culture and PCR-DGGE methods, while it was only identified in fish treated with 2.5 x 10(5) CFU g(-1) at 56 days by PCR-DGGE. Supplementation of dietary B. subtilis C-3102 altered the autochthonous gut bacterial communities, significantly increased (P < 0.05) the total amounts of adhesive viable bacteria, induced upregulation of intestinal cytokine expression (IL-1b, TGF-beta and TNF-alpha) and downregulation of intestinal HSP70. Thus dietary supplement of B. subtilis C-3102 at low dose (10(5) CFU g(-1)) is beneficial to tilapia health. (C) 2013 Elsevier B. V. All rights reserved.

引用

页码：125 / 130

页数：6

共 32 条

[1] Effects of dietary supplementation of Bacillus subtilis and fructooligosaccharide on growth performance, survival, non-specific immune response and disease resistance of juvenile large yellow croaker, Larimichthys crocea [J].