Heritable genome editing in C. elegans via a CRISPR-Cas9 system

被引：667

作者：

Friedland, Ari E. ^{[1
]}

Tzur, Yonatan B. ^{[1
]}

Esvelt, Kevin M. ^{[2
]}

Colaiacovo, Monica P. ^{[1
]}

Church, George M. ^{[1
,2
]}

Calarco, John A. ^{[3
]}

机构：

[1] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[2] Harvard Univ, Wyss Inst Biol Inspired Engn, Cambridge, MA 02138 USA

[3] Harvard Univ, Fac Arts & Sci, Ctr Syst Biol, Cambridge, MA 02138 USA

来源：

NATURE METHODS | 2013年 / 10卷 / 08期

基金：

美国国家卫生研究院;

关键词：

CAENORHABDITIS-ELEGANS; GENE;

D O I：

10.1038/nmeth.2532

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

引用

页码：741 / +

页数：5

共 25 条

[1]

BRENNER S, 1974, GENETICS, V77, P71

[2] Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease [J].

Cho, Seung Woo ;

Kim, Sojung ;

Kim, Jong Min ;

Kim, Jin-Soo .

NATURE BIOTECHNOLOGY, 2013, 31 (03) :230-232

[3] Multiplex Genome Engineering Using CRISPR/Cas Systems [J].

Cong, Le ;

Ran, F. Ann ;

Cox, David ;

Lin, Shuailiang ;

Barretto, Robert ;

Habib, Naomi ;

Hsu, Patrick D. ;

Wu, Xuebing ;

Jiang, Wenyan ;

Marraffini, Luciano A. ;

Zhang, Feng .

SCIENCE, 2013, 339 (6121) :819-823

[4] Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems [J].

DiCarlo, James E. ;

Norville, Julie E. ;

Mali, Prashant ;

Rios, Xavier ;

Aach, John ;

Church, George M. .

NUCLEIC ACIDS RESEARCH, 2013, 41 (07) :4336-4343

[5] Improved Mos1-mediated transgenesis in C. elegans [J].

Frokjaer-Jensen, Christian ;

Davis, M. Wayne ;

Ailion, Michael ;

Jorgensen, Erik M. .

NATURE METHODS, 2012, 9 (02) :117-118

[6] MUTATIONAL ANALYSIS OF THE TRANSCRIPTION START SITE OF THE YEAST TRNA(3)(LEU) GENE [J].

FRUSCOLONI, P ;

ZAMBONI, N ;

PANETTA, G ;

DEPAOLIS, A ;

TOCCHINIVALENTINI, GP .

NUCLEIC ACIDS RESEARCH, 1995, 23 (15) :2914-2918

[7] Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria [J].

Gasiunas, Giedrius ;

Barrangou, Rodolphe ;

Horvath, Philippe ;

Siksnys, Virginijus .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (39) :E2579-E2586

[8]

Gibson DG, 2009, NAT METHODS, V6, P343, DOI [10.1038/NMETH.1318, 10.1038/nmeth.1318]

[9] Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease [J].

Gratz, Scott J. ;

Cummings, Alexander M. ;

Nguyen, Jennifer N. ;

Hamm, Danielle C. ;

Donohue, Laura K. ;

Harrison, Melissa M. ;

Wildonger, Jill ;

O'Connor-Giles, Kate M. .

GENETICS, 2013, 194 (04) :1029-+

[10] Efficient genome editing in zebrafish using a CRISPR-Cas system [J].

Hwang, Woong Y. ;

Fu, Yanfang ;

Reyon, Deepak ;

Maeder, Morgan L. ;

Tsai, Shengdar Q. ;

Sander, Jeffry D. ;

Peterson, Randall T. ;

Yeh, J-R Joanna ;

Joung, J. Keith .

NATURE BIOTECHNOLOGY, 2013, 31 (03) :227-229

← 1 2 3 →