Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities

被引:65
|
作者
Ainsworth, TD [1 ]
Fine, M
Blackall, LL
Hoegh-Guldberg, O
机构
[1] Univ Queensland, Ctr Marine Studies, St Lucia, Qld 4072, Australia
[2] Univ Haifa, Leon Recanati Inst Maritime Studies, IL-31905 Haifa, Israel
[3] Univ Queensland, Adv Wastewater Management Ctr, Brisbane, Qld 4072, Australia
关键词
D O I
10.1128/AEM.72.4.3016-3020.2006
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. This study overcomes these limitations by combining FISH and spectral imaging.
引用
收藏
页码:3016 / 3020
页数:5
相关论文
共 50 条
  • [41] Locality Effect of Coral-Associated Bacterial Community in the Kuroshio Current From Taiwan to Japan
    Yang, Shan-Hua
    Tseng, Ching-Hung
    Lo, Hsueh-Ping
    Chiang, Pei-Wen
    Chen, Hsing-Ju
    Shiu, Jia-Ho
    Lai, Hung-Chun
    Tandon, Kshitij
    Isomura, Naoko
    Mezaki, Takuma
    Yamamoto, Hiromi
    Tang, Sen-Lin
    FRONTIERS IN ECOLOGY AND EVOLUTION, 2020, 8
  • [42] Coral-Associated Bacterial Assemblages: Current Knowledge and the Potential for Climate-Driven Impacts
    Mouchka, Morgan E.
    Hewson, Ian
    Harvell, C. Drew
    INTEGRATIVE AND COMPARATIVE BIOLOGY, 2010, 50 (04) : 662 - 674
  • [43] Impacts of the Deepwater Horizon oil spill on deep-sea coral-associated sediment communities
    Demopoulos, Amanda W. J.
    Bourque, Jill R.
    Cordes, Erik
    Stamler, Katherine M.
    MARINE ECOLOGY PROGRESS SERIES, 2016, 561 : 51 - 68
  • [45] Functional gene composition and metabolic potential of deep-sea coral-associated microbial communities
    Zoe A. Pratte
    Frank J. Stewart
    Christina A. Kellogg
    Coral Reefs, 2023, 42 : 1011 - 1023
  • [46] Digital mapping of bacterial artificial chromosomes by fluorescence in situ hybridization
    Jackson, SA
    Dong, FG
    Jiang, JM
    PLANT JOURNAL, 1999, 17 (05): : 581 - 587
  • [47] Functional gene composition and metabolic potential of deep-sea coral-associated microbial communities
    Pratte, Zoe A. A.
    Stewart, Frank J. J.
    Kellogg, Christina A. A.
    CORAL REEFS, 2023, 42 (05) : 1011 - 1023
  • [48] Coral-Associated Bacterial Diversity Is Conserved across Two Deep-Sea Anthothela Species
    Lawler, Stephanie N.
    Kellogg, Christina A.
    France, Scott C.
    Clostio, Rachel W.
    Brooke, Sandra D.
    Ross, Steve W.
    FRONTIERS IN MICROBIOLOGY, 2016, 7
  • [49] Bacterial Communities in Coral Offspring Vary Between in situ and ex situ Spawning Environments
    Shiu, Jia-Ho
    Lin, Che-Hung
    Mulla, Aziz Jabir
    Dang, Viet Do Hung
    Fong, Chia-Ling
    Nozawa, Yoko
    FRONTIERS IN MARINE SCIENCE, 2022, 9
  • [50] Living with marginal coral communities: Diversity and host-specificity in coral-associated barnacles in the northern coral distribution limit of the East China Sea
    Chan, Benny K. K.
    Xu, Guang
    Kim, Hyun Kyong
    Park, Jin-Ho
    Kim, Won
    PLOS ONE, 2018, 13 (05):