Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities

被引：65

作者：

Ainsworth, TD ^{[1
]}

Fine, M

Blackall, LL

Hoegh-Guldberg, O

机构：

[1] Univ Queensland, Ctr Marine Studies, St Lucia, Qld 4072, Australia

[2] Univ Haifa, Leon Recanati Inst Maritime Studies, IL-31905 Haifa, Israel

[3] Univ Queensland, Adv Wastewater Management Ctr, Brisbane, Qld 4072, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 04期

关键词：

D O I：

10.1128/AEM.72.4.3016-3020.2006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. This study overcomes these limitations by combining FISH and spectral imaging.

引用

页码：3016 / 3020

页数：5

共 42 条

[1] The identification of microorganisms by fluorescence in situ hybridisation [J].

Amann, R ;

Fuchs, BM ;

Behrens, S .

CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (03) :231-236

[2] In situ visualization of high genetic diversity in a natural microbial community [J].

Amann, R ;

Snaidr, J ;

Wagner, M ;

Ludwig, W ;

Schleifer, KH .

JOURNAL OF BACTERIOLOGY, 1996, 178 (12) :3496-3500

[3] PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].

AMANN, RI ;

LUDWIG, W ;

SCHLEIFER, KH .

MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169

[4] COMBINATION OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES WITH FLOW-CYTOMETRY FOR ANALYZING MIXED MICROBIAL-POPULATIONS [J].

AMANN, RI ;

BINDER, BJ ;

OLSON, RJ ;

CHISHOLM, SW ;

DEVEREUX, R ;

STAHL, DA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (06) :1919-1925

[5] Evaluation of spectral imaging for plant cell analysis [J].

Berg, RH .

JOURNAL OF MICROSCOPY-OXFORD, 2004, 214 :174-181

[6] RNA interference in the moss Physcomitrella patens [J].

Bezanilla, M ;

Pan, A ;

Quatrano, RS .

PLANT PHYSIOLOGY, 2003, 133 (02) :470-474

[7] Factors influencing the detection of bacterial cells using fluorescence in situ hybridization (FISH): A quantitative review of published reports [J].

Bouvier, T ;

del Giorgio, PA .

FEMS MICROBIOLOGY ECOLOGY, 2003, 44 (01) :3-15

[8] Microbial communities associated with skeletal tumors on Porites compressa [J].

Breitbart, M ;

Bhagooli, R ;

Griffin, S ;

Johnston, I ;

Rohwer, F .

FEMS MICROBIOLOGY LETTERS, 2005, 243 (02) :431-436

[9] Histopathological methods for the investigation of microbial communities associated with disease lesions in reef corals [J].

Bythell, JC ;

Barer, MR ;

Cooney, RP ;

Guest, JR ;

O'Donnell, AG ;

Pantos, O ;

Le Tissier, MDA .

LETTERS IN APPLIED MICROBIOLOGY, 2002, 34 (05) :359-364

[10] The domain-specific probe EUB338 is insufficient for the detection of all Bacteria:: Development and evaluation of a more comprehensive probe set [J].

Daims, H ;

Brühl, A ;

Amann, R ;

Schleifer, KH ;

Wagner, M .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1999, 22 (03) :434-444

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