Melanins and melanogenesis: methods, standards, protocols

被引：366

作者：

d'Ischia, Marco ^{[1
]}

Wakamatsu, Kazumasa ^{[2
]}

Napolitano, Alessandra ^{[1
]}

Briganti, Stefania ^{[3
]}

Garcia-Borron, Jose-Carlos ^{[4
]}

Kovacs, Daniela ^{[3
]}

Meredith, Paul ^{[5
]}

Pezzella, Alessandro ^{[1
]}

Picardo, Mauro ^{[3
]}

Sarna, Tadeusz ^{[6
]}

Simon, John D. ^{[7
]}

Ito, Shosuke ^{[2
]}

机构：

[1] Univ Naples Federico II, Dept Chem Sci, Naples, Italy

[2] Fujita Hlth Univ, Sch Hlth Sci, Dept Chem, Toyoake, Aichi, Japan

[3] San Gallicano Dermatol Inst, Cutaneous Physiopathol Lab, Rome, Italy

[4] Univ Murcia, Sch Med, Dept Biochem & Mol Biol, Murcia, Spain

[5] Univ Queensland, Sch Math & Phys, Ctr Organ Photon & Elect, Brisbane, Qld, Australia

[6] Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Biophys, Krakow, Poland

[7] Univ Virginia, Dept Chem, Charlottesville, VA USA

来源：

PIGMENT CELL & MELANOMA RESEARCH | 2013年 / 26卷 / 05期

关键词：

melanin analysis; melanin synthesis; melanin isolation; melanin spectral characterization; melanogenesis enzymes; method standardization; CELL LUNG-CANCER; EML4-ALK FUSION GENE; MALIGNANT-MELANOMA; THERAPEUTIC TARGET; COLON-CANCER; MUTATION; BRAF; INHIBITION; VARIANTS; CHINESE;

D O I：

10.1111/pcmr.12121

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Despite considerable advances in the past decade, melanin research still suffers from the lack of universally accepted and shared nomenclature, methodologies, and structural models. This paper stems from the joint efforts of chemists, biochemists, physicists, biologists, and physicians with recognized and consolidated expertise in the field of melanins and melanogenesis, who critically reviewed and experimentally revisited methods, standards, and protocols to provide for the first time a consensus set of recommended procedures to be adopted and shared by researchers involved in pigment cell research. The aim of the paper was to define an unprecedented frame of reference built on cutting-edge knowledge and state-of-the-art methodology, to enable reliable comparison of results among laboratories and new progress in the field based on standardized methods and shared information.

引用

页码：616 / 633

页数：18

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