Exogenous menadione sodium bisulphite alleviates detrimental effects of alkaline stress on wheat (Triticum aestivum L.)

Menadione sodium bisulphite (MSB) is known to augment plant defense responses against abiotic and biotic stresses. Wheat is an essential cereal with significant sensitivity to alkaline stress. The present study investigated the effects of MSB seed priming (5 and 10 mM) in alleviating the damaging effects of alkaline stress on hydroponically grown wheat cultivars (salt-sensitive cv. MH-97 and salt-tolerant cv. Millat-2011). Our findings revealed a significant reduction in growth, chlorophyll contents, total soluble proteins, free amino acids, K+, Ca2+, P, and K+/Na+ in wheat cultivars under alkaline stress. In contrast, a noteworthy accretion in lipid peroxidation, H2O2 production, proline levels, antioxidant enzyme activities, soluble sugars, antioxidant compounds, and Na+ levels was noticed in wheat plants grown in alkaline hydroponic medium. MSB priming significantly lowered chlorophyll degradation, Na+ levels, and osmolyte accumulation. Further, K+/Na+ ratio, antioxidant compounds, and antioxidant enzyme activities were higher in plants primed with MSB. Therefore, seed priming eminently protected plants by regulating osmotic adjustment and strengthening oxidative defense under alkaline stress. Plants administered 5 mM MSB as seed priming manifested better tolerance to alkaline stress.