Simultaneous detection of six RNA plant viruses affecting tomato crops using a single digoxigenin-labelled polyprobe

被引：25

作者：

Aparicio, Frederic ^{[3
]}

Soler, Salvador ^{[1
]}

Aramburu, Jose ^{[2
]}

Galipienso, Luis ^{[2
]}

Nuez, Fernando ^{[1
]}

Pallas, Vicente ^{[3
]}

Lopez, Carmelo ^{[1
]}

机构：

[1] Univ Politecn Valencia, Inst Conservac & Mejora Agrodiversidad Valenciana, Valencia 46022, Spain

[2] IRTA, Barcelona 08348, Spain

[3] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2009年 / 123卷 / 01期

关键词：

Diagnosis; Dig-RNA polyprobe; Tissue-printing; Tomato; NONISOTOPIC MOLECULAR HYBRIDIZATION;

D O I：

10.1007/s10658-008-9347-5

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A polyprobe for the simultaneous detection by non-isotopic molecular hybridisation has been developed to detect any of the following six viruses causing important economic losses in tomato crops: Tomato spotted wilt virus, Tomato mosaic virus, Pepino mosaic virus, Cucumber mosaic virus, Potato Y virus and Parietaria mottle virus. The polyprobe detected all six viruses with similar sensitivity to that obtained using individual riboprobes. In addition, we evaluated the possible use of the tissue-printing as a sample preparation technique applied to routine diagnosis of tomato plants with the polyprobe.

引用

页码：117 / 123

页数：7

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