A novel mastrevirus infecting wheat in India

被引:14
作者
Kumar, Jitendra [1 ]
Singh, Sudhir P. [1 ]
Kumar, Jitesh [1 ]
Tuli, Rakesh [1 ]
机构
[1] Natl Agri Food Biotechnol Inst, Mohali 160071, Punjab, India
关键词
DWARF VIRUS; BARLEY;
D O I
10.1007/s00705-012-1359-y
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A new mastrevirus (family Geminivridae) infecting wheat in India was detected by rolling-circle amplification (RCA). The complete nucleotide sequence of the virus was determined to be 2783 bp long. Analysis of the nucleotide sequence revealed identity and a genome organisation typical of a mastrevirus. An identical virus was detected in the candidate insect vector (leafhopper) collected from the field. Agroinoculation of young wheat plants with an infectious clone of the virus resulted in dwarfing of plants, identical to what was observed in the field, confirming that this novel virus was the causative agent of the disease. Considering the low degree of sequence identity to any known mastrevirus, the virus described here is suggested to be a member of a new species. Based on symptoms, we propose the name "wheat dwarf India virus".
引用
收藏
页码:2031 / 2034
页数:4
相关论文
共 9 条
[1]   Widespread occurrence and molecular characterization of Wheat dwarf virus in Iran [J].
Behjatnia, S. A. A. ;
Afsharifar, A. R. ;
Tahan, V. ;
Motlagh, M. H. Amid ;
Gandomani, O. Eini ;
Niazi, A. ;
Izadpanah, K. .
AUSTRALASIAN PLANT PATHOLOGY, 2011, 40 (01) :12-19
[2]   Development of a multiplexed PCR detection method for Barley and Cereal yellow dwarf viruses, Wheat spindle streak virus, Wheat streak mosaic virus and Soil-borne wheat mosaic virus [J].
Deb, Mahua ;
Anderson, Joseph M. .
JOURNAL OF VIROLOGICAL METHODS, 2008, 148 (1-2) :17-24
[3]   Geminivirus strain demarcation and nomenclature [J].
Fauquet, C. M. ;
Briddon, R. W. ;
Brown, J. K. ;
Moriones, E. ;
Stanley, J. ;
Zerbini, M. ;
Zhou, X. .
ARCHIVES OF VIROLOGY, 2008, 153 (04) :783-821
[4]   OUTBREAK OF MAV-TYPE BARLEY YELLOW DWARF VIRUS ON WHEAT IN THE GARHWAL HILLS IN INDIA [J].
KHETARPAL, RK ;
KUMAR, J ;
BEUVE, M ;
PARAKH, DB ;
NATH, R .
PLANT PATHOLOGY, 1994, 43 (02) :415-416
[5]   A novel sugarcane-infecting mastrevirus from South Africa [J].
Lawry, Robert ;
Martin, Darren P. ;
Shepherd, Dionne N. ;
van Antwerpen, Tania ;
Varsani, Arvind .
ARCHIVES OF VIROLOGY, 2009, 154 (10) :1699-1703
[6]   Widely Conserved Recombination Patterns among Single-Stranded DNA Viruses [J].
Lefeuvre, P. ;
Lett, J. -M. ;
Varsani, A. ;
Martin, D. P. .
JOURNAL OF VIROLOGY, 2009, 83 (06) :2697-2707
[7]   RDP3: a flexible and fast computer program for analyzing recombination [J].
Martin, Darren P. ;
Lemey, Philippe ;
Lott, Martin ;
Moulton, Vincent ;
Posada, David ;
Lefeuvre, Pierre .
BIOINFORMATICS, 2010, 26 (19) :2462-+
[8]   USE OF GROUP-SPECIFIC PRIMERS AND THE POLYMERASE CHAIN-REACTION FOR THE DETECTION AND IDENTIFICATION OF LUTEOVIRUSES [J].
ROBERTSON, NL ;
FRENCH, R ;
GRAY, SM .
JOURNAL OF GENERAL VIROLOGY, 1991, 72 :1473-1477
[9]   MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0 [J].
Tamura, Koichiro ;
Dudley, Joel ;
Nei, Masatoshi ;
Kumar, Sudhir .
MOLECULAR BIOLOGY AND EVOLUTION, 2007, 24 (08) :1596-1599