Folate-PEG-superparamagnetic iron oxide nanoparticles for lung cancer imaging

被引：93

作者：

Yoo, Mi-Kyong ^{[2
,3
,4
]}

Park, In-Kyu ^{[5
,6
]}

Lim, Hwang-Tae ^{[1
]}

Lee, Sang-Joon ^{[5
,6
]}

Jiang, Hu-Lin ^{[1
]}

Kim, You-Kyoung ^{[2
,3
]}

Choi, Yun-Jaie ^{[2
,3
]}

Cho, Myung-Haing ^{[1
,7
,8
]}

Cho, Chong-Su ^{[2
,3
]}

机构：

[1] Seoul Natl Univ, Coll Vet Med, Toxicol Lab, Seoul 151742, South Korea

[2] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 151921, South Korea

[3] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 151921, South Korea

[4] Kyung Hee Univ, Dept Adv Mat Engn Informat & Elect, Yongin 446701, South Korea

[5] Chonnam Natl Univ, Sch Med, Dept Biomed Sci, Kwangju 501746, South Korea

[6] Ctr Biomed Human Resources, BK Program 21, Kwangju 501746, South Korea

[7] Seoul Natl Univ, Adv Inst Convergence Technol, Grad Sch Convergence Sci & Technol, Dept Nano Sci & Technol, Suwon 443270, South Korea

[8] Seoul Natl Univ, Grad Grp Tumor Biol, Seoul 151742, South Korea

来源：

ACTA BIOMATERIALIA | 2012年 / 8卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

Magnetic nanoparticles; Folate; Poly(ethylene glycol); Lung cancer; Imaging; MAGNETIC NANOPARTICLES; CELL-LINES; RECEPTOR; THERAPY; PARTICLES; TISSUES;

D O I：

10.1016/j.actbio.2012.04.029

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

While superparamagnetic iron oxide nanoparticles (SPIONs) have been widely used in biomedical applications, rapid blood clearance, instability and active targeting of the SPIONs limit their availability for clinical trials. This work was aimed at developing stable and lung cancer targeted SPIONs. For this purpose firstly folk acid (FA)-conjugated poly(ethylene glycol) (FA-PEG) was synthesized, and FA-PEG-SPIONs were subsequently prepared by the reaction of FA-PEG with aminosilane-immobilized SPIONs. FA-PEG-SPIONs were labeled with Cy5.5 for optical imaging. The intracellular uptake of FA-PEG-SPIONs-Cy5.5 was evaluated in KB cells and lung cancer model mice to confirm active targeting. The sizes of the FA-PEG-SPIONs were little changed after up to 8 weeks at 4 degrees C, suggestive of very stable particle sizes. The results of fluorescent flow cytometry and confocal laser scanning microscopy suggest that the intracellular uptake of FA-PEG-SPIONs-Cy5.5 was greatly inhibited by pre-treatment with free folic acid, indicative of receptor-mediated endocytosis. Stronger optical imaging was observed in the lung cancer model mice for FA-PEG-SPIONs-Cy5.5 than PEG-SPIONs-Cy5.5 6 and 24 h post-injection through the tail vein, due to receptor-mediated endocytosis. (c) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：3005 / 3013

页数：9

共 29 条

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