Activin A/Smads signaling pathway negatively regulates Oxygen Glucose Deprivation-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells

被引：29

作者：

Xue, Long-Xing ^{[1
]}

Xu, Zhong-Hang ^{[2
]}

Wang, Jiao-Qi ^{[1
]}

Cui, Yang ^{[1
]}

Liu, Hong -Yu ^{[1
]}

Liang, Wen-Zhao ^{[1
]}

Ji, Qiu-Ye ^{[3
]}

He, Jin-Ting ^{[1
]}

Shao, Yan-Kun ^{[1
]}

Mang, Jing ^{[1
]}

Xu, Zhong-Xin ^{[1
]}

机构：

[1] Jilin Univ, China Japan Union Hosp, Dept Neurol, 126 Xiantai St, Changchun 130012, Peoples R China

[2] Jilin Univ, Clin Med Norman Bethune Med Dept, Changchun 130012, Jilin Province, Peoples R China

[3] Jilin Univ, China Japan Union Hosp, Res Ctr, 126 Xiantai St, Changchun 130012, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 480卷 / 03期

关键词：

Activin A/Smads; Autophagy; JNK; p38; Oxygen Glucose Deprivation (OGD); FOCAL CEREBRAL-ISCHEMIA; TRANSIENT FOREBRAIN ISCHEMIA; ACTIVATED PROTEIN-KINASES; BRAIN-INJURY; RAT MODEL; DEATH; CONTRIBUTES; RECEPTORS; CANCER; MICE;

D O I：

10.1016/j.bbrc.2016.10.050

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Activin A (Act A), a member of the transforming growth factor-beta (TGF-beta), reduces neuronal apoptosis during cerebral ischemia through Act A/Smads signaling pathway. However, little is known about the effect of Act A/Smads pathway on autophagy in neurons. Here, we found that oxygen-glucose deprivation (OGD)-induced autophagy was suppressed by exogenous Act A in a concentration-dependent manner and enhanced by Act A/Smads pathway inhibitor (ActRIIA-Ab) in neuronal PC12 cells. These results indicate that Act A/Smads pathway negatively regulates autophagy in OGD-treated PC12 cells. In addition, we found that c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein (MAP) kinase pathways are involved in the OGD-induced autophagy. The activation of JNK and p38 MAPK pathways in OGD-treated PC12 cells was suppressed by exogenous Act A and enhanced by ActRIIA-Ab. Together, our results suggest that Act A/Smads signaling pathway negatively regulates OGD-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：355 / 361

页数：7

共 38 条

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