Identification of cDNAs encoding allatotropin and allatotropin-like peptides from the silkworm, Bombyx mori

被引：24

作者：

Nagata, Shinji ^{[1
]}

Matsumoto, Sumihiro ^{[1
]}

Mizoguchi, Akira ^{[2
]}

Nagasawa, Hiromichi ^{[1
]}

机构：

[1] Univ Tokyo, Dept Appl Biol Chem, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[2] Nagoya Univ, Div Biol Sci, Grad Sch Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan

来源：

PEPTIDES | 2012年 / 34卷 / 01期

关键词：

Allatotropin; Allatotropin-like peptide; Bombyx mori; cDNA cloning; MANDUCA-SEXTA ALLATOTROPIN; JUVENILE-HORMONE BIOSYNTHESIS; LASER-SCANNING MICROSCOPY; FRONTAL GANGLION; HELIOTHIS-VIRESCENS; CORPORA ALLATA; NEUROPEPTIDE COLOCALIZATION; MOLECULAR CHARACTERIZATION; ALLATOREGULATORY PEPTIDES; LACANOBIA-OLERACEA;

D O I：

10.1016/j.peptides.2012.01.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNAs encoding allatotropin (AT) and allatotropin-like peptides (ATLPs) were isolated from the silkworm, Bombyx mori. Similar to those of the tobacco hornworm, Manduca sexta, four peptides (AT, ATLP1, ATLP2, and ATLP3) are present in three different variants generated by alternative splicing. RTPCR analyses showed that these splice variants are expressed in the central nervous system with differing expression patterns in each ganglion. Immunohistochemistry using an anti-AT antibody confirmed that AT-expressing cells were located in these central nervous ganglia as well as in two large anterior cells of the frontal ganglia. Injection of synthetic AT and ATLP-1 into B. mori larvae increased the latency to feed, indicating that AT and ATLP might function in the regulation of feeding behavior in B. mori. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：98 / 105

页数：8