Noninvasive fetal RHD genotyping to guide targeted anti-D prophylaxis-an external quality assessment workshop

被引：17

作者：

Clausen, Frederik Banch ^{[1
,2
]}

Barrett, Angela Natalie ^{[1
,2
]}

Akkok, Cigdem Akalin ^{[3
]}

Armstrong-Fisher, Sylvia ^{[4
]}

Bergstrom, Karolina Danielsson ^{[5
]}

Boggione, Carolina Trucco ^{[6
]}

Baevre, Mette Silihagen ^{[3
]}

Choolani, Mahesh ^{[7
]}

Christiansen, Mette ^{[8
]}

Cotorruelo, Carlos ^{[6
]}

Drnovsek, Tadeja Dovc ^{[9
]}

Finning, Kirstin ^{[10
]}

Guz, Katarzyna ^{[11
]}

de Haas, Masja ^{[12
]}

Haimila, Katri ^{[13
]}

Halldorsdottir, Anna Margret ^{[14
]}

Hellberg, Asa ^{[15
,16
]}

Henny, Christine ^{[17
]}

Holmertz, Camilla ^{[18
]}

Al Houghton, Jayne ^{[19
]}

Hyland, Catherine ^{[20
]}

Jakobsen, Marianne Antonius ^{[21
]}

Kvitland, Mona Andersen ^{[22
]}

Lambert, Mark ^{[23
]}

Legler, Tobias J. ^{[24
]}

Liew, Yew-Wah ^{[20
]}

Muniz-Diaz, Eduardo ^{[25
]}

Mortberg, Anette ^{[26
,27
]}

Niederhauser, Christoph ^{[17
]}

Nogues, Nuria ^{[25
]}

Nystrom, Sofia ^{[18
]}

Olsson, Martin L. ^{[15
,16
]}

Orzinska, Agnieszka ^{[11
]}

Parks, Michael ^{[28
]}

Rietkotter, Eva ^{[29
]}

Ryan, Helen ^{[23
]}

Sachs, Ulrich J. ^{[30
]}

van der Schoot, Ellen ^{[31
]}

Silcock, Lee ^{[28
]}

Steffensen, Rudi ^{[32
]}

Sulin, Kati ^{[13
]}

Sorensen, Anne Solling ^{[33
]}

Tarrant, Sarah ^{[10
]}

Thorlacius, Steinunn ^{[14
]}

Wienzek-Lischka, Sandra ^{[30
]}

Wikman, Agneta ^{[26
,27
]}

Wulf-Johansson, Helle ^{[33
]}

Zupan, Mojca ^{[9
]}

Dziegiel, Morten Hanefeld ^{[34
,35
]}

机构：

[1] Copenhagen Univ Hosp, Rigshosp, Dept Clin Immunol, KI 2034,Blegdamsvej 9, DK-2100 Copenhagen O, Denmark

[2] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Obstet & Gynaecol, Singapore, Singapore

[3] Oslo Univ Hosp, Dept Immunol & Transfus Med, Oslo, Norway

[4] Scottish Natl Blood Transfus Serv, Dept Mol Immunohaematol, Aberdeen, Scotland

[5] Orebro Univ Hosp, Dept Clin Immunol & Transfus Med, Orebro, Sweden

[6] Univ Nacl Rosario, CONICET, IDICER, Lab Inmunohematol, Rosario, Santa Fe, Argentina

[7] Natl Univ Singapore City, Yong Loo Lin Sch Med, Dept Obstet & Gynaecol, Singapore, Singapore

[8] Aarhus Univ, Dept Clin Immunol, Aarhus, Denmark

[9] Blood Transfus Ctr Slovenia, Ljubljana, Slovenia

[10] NHS Blood & Transplant, Filton, England

[11] Inst Hematol & Transfus Med, Dept Immunohematol & Immunol Transfus Med, Warsaw, Poland

[12] Sanquin Blood Supply Fdn, Amsterdam, Netherlands

[13] Finnish Red Cross Blood Serv, Helsinki, Finland

[14] Landspitali Univ Hosp, Blood Bank, Reykjavik, Iceland

[15] Lund Univ, Dept Clin Immunol & Transfus Med, Nord Reference Lab Genom Blood Grp Anal, LabMed,Off Med Serv,Reg Skane, Lund, Sweden

[16] Lund Univ, Dept Hematol & Transfus Med, Lund, Sweden

[17] Interreg Blood Transfus SRC, Bern, Switzerland

[18] Linkoping Univ, Dept Clin & Expt Med, Clin Immunol & Transfus Med, Linkoping, Sweden

[19] Royal Devon & Exeter NHS Fdn Trust, Dept Mol Genet, Exeter, Devon, England

[20] Australian Red Cross Blood Serv, Red Cell Reference Lab, Brisbane, Qld, Australia

[21] Odense Univ Hosp, Dept Clin Immunol, Odense, Denmark

[22] St Olavs Hosp, Dept Med Biochem, Trondheim, Norway

[23] Natl Blood Ctr, Irish Blood Transfus Serv, Dublin, Ireland

[24] Georg August Univ, Univ Med Gottingen, Dept Transfus Med, Gottingen, Germany

[25] Banc Sang & Teixits, Barcelona, Spain

[26] Karolinska Univ Hosp, Dept Clin Immunol & Transfus Med, Stockholm, Sweden

[27] Karolinska Inst, Stockholm, Sweden

[28] Nonacus Ltd, Birmingham, W Midlands, England

[29] LADR GmbH MVZ Dr Kramer & Kollegen, Geesthacht, Germany

[30] Justus Liebig Univ, Inst Clin Immunol & Transfus Med, Giessen, Germany

[31] Sanquin Res CLB, Amsterdam, Netherlands

[32] Aalborg Univ Hosp, Dept Clin Immunol, Aalborg, Denmark

[33] Naestved Hosp, Dept Clin Immunol, Naestved, Denmark

[34] Copenhagen Univ Hosp, Rigshosp, Dept Clin Immunol, Copenhagen, Denmark

[35] Univ Copenhagen, Inst Clin Med IKM, Copenhagen, Denmark

来源：

VOX SANGUINIS | 2019年 / 114卷 / 04期

关键词：

fetal RHD genotyping; cell-free fetal DNA; external quality assessment; Rh prophylaxis; SANGUINIS INTERNATIONAL FORUM; PREGNANT-WOMEN; DISEASE; PLASMA; DNA;

D O I：

10.1111/vox.12768

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background and Objectives Fetal RHD genotyping of cell-free fetal DNA from RhD-negative pregnant women can be used to guide targeted antenatal and postnatal anti-D prophylaxis for the prevention of RhD immunization. To assure the quality of clinical testing, we conducted an external quality assessment workshop with the participation of 28 laboratories. Materials and Methods Aliquots of pooled maternal plasma were sent to each laboratory. One sample was positive, and the second sample was negative for fetal RHD, verified by pre-workshop testing using quantitative real-time PCR (qPCR) analysis of RHD exons 4, 5, 7 and 10. Plasma samples were shipped at room temperature. A reporting scheme was supplied for data collection, including questions regarding the methodological setup, results and clinical recommendations. Different methodological approaches were used, all employing qPCR with a total of eight different combinations of RHD exon targets. The samples were tested blindly. Results Fetal RHD genotyping was performed with no false-negative and no false-positive results. One inconclusive result was reported for the RHD-positive sample, and four inconclusive results were reported for the RHD-negative sample. All clinical conclusions were satisfactory. Conclusion This external quality assessment workshop demonstrates that despite the different approaches taken to perform the clinical assays, fetal RHD genotyping is a reliable laboratory assay to guide targeted use of Rh prophylaxis in a clinical setting.

引用

页码：386 / 393

页数：8

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