Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

被引:17
|
作者
Kubo, Yuji [1 ]
Inaoka, Takashi [2 ]
Hachiya, Tsuyoshi [3 ,4 ]
Miyake, Masayuki [3 ]
Hase, Sumitaka [3 ]
Nakagawa, Rikio [1 ]
Hasegawa, Hiromasa [1 ]
Funane, Kazumi [2 ]
Sakakibara, Yasubumi [3 ]
Kimura, Keitarou [2 ]
机构
[1] Ind Technol Inst Ibaraki Prefecture, Ibaraki 3113195, Japan
[2] NARO, NFRI, Div Appl Microbiol, Tsukuba, Ibaraki 3058642, Japan
[3] Keio Univ, Dept Biosci & Informat, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan
[4] Iwate Med Univ, Iwate Med Megabank Org, Shiwa, Iwate 0283694, Japan
关键词
Bacillus subtilis; Rifampicin resistance; Colored soybean; Exoenzyme production; Natto; RNA-POLYMERASE MUTATION; PROTEIN; NEOTREHALOSADIAMINE; EXPRESSION; COMA; DEGU;
D O I
10.1016/j.jbiosc.2012.12.012
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the beta-subunit of the RNA polymerase. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码:654 / 657
页数:4
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