Distribution of parkin in the adult rat brain

被引：14

作者：

D'Agata, V

Zhao, WQ

Pascale, A

Zohar, O

Scapagnini, G

Cavallaro, S

机构：

[1] W Virginia Univ, Blanchette Rockefeller Neurosci Inst, Rockville, MD 20850 USA

[2] Italian Natl Res Council, Inst Bioimaging & Pathophysiol Cent Nervous Syst, Catania, Italy

来源：

PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY | 2002年 / 26卷 / 03期

关键词：

autosomal recessive juvenile parkinsonism; Parkinson's disease; central nervous system; distribution; immunohistochemistry;

D O I：

10.1016/S0278-5846(01)00301-3

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

A mutation in the parkin gene has been identified as the cause for an autosomal recessively inherited form of Parkinson's disease (PD). The authors have recently isolated the mRNA coding for the rat homolog of parkin and showed its widespread expression in the central nervous system (CNS) by in situ hybridization. In the present study, we investigated the distribution of parkin in the rat CNS with a polyclonal antibody that reacts with a similar to52-kDa protein, mainly localized in the cytoplasm and corresponding to the predicted molecular mass of parkin. Immunohistochemistry on adult rat brain sections showed a widespread distribution of parkin. This included labeling of cell bodies, nuclei as well as processes in the hippocampus, cerebral cortex, cerebellum, and several nuclei in the brainstem. The regional expression of parkin-immunoreactivity (IR) correlated well with the parkin-mRNA levels assessed by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). This study provides the detailed analysis of the regional and cellular distribution of parkin in the rat brain and may be useful in elucidating its pathophysiological role. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：519 / 527

页数：9

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