MiR-29 mediates TGF1-induced extracellular matrix synthesis through activation of PI3K-AKT pathway in human lung fibroblasts

TGF1 is very important in the synthesis and degradation of extracellular matrix, and also in the mediation of human lung fibroblasts proliferation, and miR-29 plays an important role in this process. To explore the interactions of miR-29 family members and TGF1, the effects of transforming growth factor TGF1 on the expression of miR-29 and whether miR-29 is involved in pro-survival signaling pathways mediated by TGF1 were examined in human lung fibroblasts. Treatment of the human embryonic lung fibroblast cell line IMR90 with TGF1 caused a decrease in expression of miR-29a/b/c by real-time PCR analysis. TGF1 stimulation increased cell proliferation, colony formation and up-regulated expression of COL1A1; transfecting with miR-29a/b/c mimics reverse TGF1-induced phenotype changes in IMR90 cells. Western blot analyses showed that TGF1 treatment unchanged total protein expression levels of PI3K or AKT, but the expression levels of p-PI3K, p-AKT, and COL1A1 were increased; and miR-19a/b/c mimics interfering blocked phosphorylation of PI3K or AKT and decreased expression of COL1A1 after TGF1 treatment. The results indicate that TGF1 beta uses the PI3k-Akt pathway in these embryonic fibroblasts and miR29 blocks this activation pathway. It indicates a novel biological function of the PI3K-Akt pathway in IMR90. Elevated expression of miR-29 may play an important role in the pathogenesis of diseases related to fibrogenic reactions in human lung fibroblasts. J. Cell. Biochem. 114: 13361342, 2013. (c) 2013 Wiley Periodicals, Inc.