Nano-electrospray tandem mass spectrometry for the analysis of neurosteroid sulphates

Neurosteroids are synthesised in the central and peripheral nervous system or are derived from peripheral sources, and act in the nervous system, In the present study we have evaluated the potential for using nano-electrospray (nano-ES) tandem mass spectrometry (MS/MS) for the structural analysis and detection of neurosteroids, in particular, steroid sulphates found in brain, Complete structural information can be obtained from 1 ng (3 pmol) of steroid sulphate, while fragment ions characteristic of the sulphate ester group can be obtained from only 3 pg (10 fmol) of sample. These values correspond to the expected quantities of steroid sulphates (e.g, pregnenolone sulphate) in about 100mg and 300 mu g of brain, respectively. Deuterated neurosteroid sulphates added to homogenised rat brain have been successfully analysed by nano-ES-MS/MS at a level of 50 pg/mg of brain, Copyright (C) 1999 John Whey & Sons, Ltd.