Sucrose biotransformation by immobilized Phaffia rhodozyma and continuous neokestose production in a packed-bed reactor

被引：5
作者：
Bie, Xiao-Ying [1 ]
Zhu, Ming-Jun [1 ,2 ]
机构：
[1] South China Univ Technol, Sch Biosci & Bioengn, Guangzhou Higher Educ Mega Ctr, Guangzhou 510006, Guangdong, Peoples R China
[2] South China Univ Technol, Guangdong Prov Key Lab Fermentat & Enzyme Engn, Guangzhou, Guangdong, Peoples R China
来源：
BIOCATALYSIS AND BIOTRANSFORMATION | 2016年 / 34卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Biotransformation; neokestose; cell immobilization; Phaffia rhodozyma; alginate-chitosan; BETA-FRUCTOFURANOSIDASE; AUREOBASIDIUM-PULLULANS; FRUCTOOLIGOSACCHARIDE PRODUCTION; NEO-FRUCTOOLIGOSACCHARIDES; ASPERGILLUS-JAPONICUS; PENICILLIUM-CITRINUM; FERMENTATION; BEADS; CELLS; NEOFRUCTOOLIGOSACCHARIDES;
D O I：
10.1080/10242422.2016.1247813
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of important production variables on the biotransformation of sucrose by immobilized cells of Phaffia rhodozyma were investigated. Cell concentration had negative effect on the maximum concentration of neokestose and the optimal concentration was 16 g/l (calculated by dry weight). The concentration of sucrose had a positive effect on the maximum concentration of neokestose within 1.170 mol/l. Elevating the reaction temperature increased the reaction rate but decreased the maximum concentration of neokestose. Sugar cane juice could be used as an inexpensive substrate for neokestose production. Additionally, a 30-d continuous neokestose production was found feasible in a packed bed reactor, indicating that the cell immobilization with chitosan-coated alginate has the potential for industrial production.
引用
收藏
页码：89 / 98
页数：10
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