Alteration of the structure and function of photosystem I by Pb2+

被引：13
|
作者：
Belatik, Ahmed [1 ]
Hotchandani, Surat [1 ]
Tajmir-Riahi, Heidar-Ali [1 ]
Carpentier, Robert [1 ]
机构：
[1] Univ Quebec Trois Rivieres, Grp Rech Biol Vegetale, Trois Rivieres, PQ G9A 5H7, Canada
来源：
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2013年 / 123卷
关键词：
Pb cation; Photosystem I; Plastocyanin; Fluorescence; XPS; Electron transport; RAY-INDUCED DECOMPOSITION; PHOTOCHEMICAL ACTIVITY; SECONDARY STRUCTURE; MASS-SPECTROMETRY; ELECTRON FLOW; HEAVY-METALS; ENERGY; PROTEINS; PLANT; CHLOROPLASTS;
D O I：
10.1016/j.jphotobiol.2013.03.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The toxic effects of Pb2+ on photosynthetic electron transport were studied in photosystem I (PSI) submembrane fractions isolated from spinach. Structural and spectroscopic analysis using FTIR, fluorescence and X-ray photoelectron spectroscopy (XPS) showed that Pb2+ constant of Kpb-PSI = 4.9 x 10(3)(+/- 0.2) M-1 and the number of bound Pb2+ cation was 0.9 per PSI complex. Pb" binding altered the protein conformation indicating a partial protein destabilization. Electron transport and P700 photooxidation/reduction measurements showed that the interaction of Pb2+ cations with PSI produced a donor side limitation of electron transport presumably due to Pb" binding to or in the vicinity of plastocyanin. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：41 / 47
页数：7
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