An Inexpensive Alternative Equipment for the Plant Material Embedding in the Paraffin under the Vacuum

被引：2

作者：

Espolador Leitao, Carlos Andre ^{[1
]}

Cortelazzo, Angelo Luiz ^{[2
]}

机构：

[1] Univ Estadual Sudoeste Bahia, Dept Ciencias Nat, BR-45083900 Vitoria Da Conquista, BA, Brazil

[2] Univ Estadual Campinas, Dept Biol Celular, BR-13083863 Campinas, SP, Brazil

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2008年 / 51卷 / 05期

关键词：

Embedding; paraffin method; plant anatomy; technique in microscopy;

D O I：

10.1590/S1516-89132008000500018

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The present work describes an equipment constructed using inexpensive material for embedding the plant material in the paraffin under the vacuum, using an oven and a vacuum pump. The equipment was tested using the samples of Rodriguezia venusta (Orchidaceae) buds embedded in paraffin where half of the samples were submitted to the vacuum by the equipment during the embedding. The material was sectioned with a rotary microtome, obtaining full series of quality sections. The control was hard to section with the microtome, obtaining damaged sections due the air bubbles, making the ribbon formation difficult. These results proved the effectiveness of the equipment, making it a practical, inexpensive and more portable solution for newly established laboratories.

引用

页码：1011 / 1014

页数：4