Unzipping Single DNA Molecules to Study Nucleosome Structure and Dynamics

被引：21

作者：

Li, Ming ^{[2
]}

Wang, Michelle D. ^{[1
,3
]}

机构：

[1] Cornell Univ, Dept Phys, Atom & Solid State Phys Lab, Ithaca, NY 14853 USA

[2] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY USA

[3] Cornell Univ, Howard Hughes Med Inst, Ithaca, NY USA

来源：

NUCLEOSOMES, HISTONES & CHROMATIN, PT B | 2012年 / 513卷

基金：

美国国家科学基金会;

关键词：

CORE PARTICLE; HIGH-AFFINITY; FORCE; HISTONES; STABILITY; CHROMATIN; RESOLUTION; ENERGY;

D O I：

10.1016/B978-0-12-391938-0.00002-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA unzipping is a powerful tool to study protein-DNA interactions at the single-molecule level. In this chapter, we provide a detailed and practical guide to performing this technique with an optical trap, using nucleosome studies as an example. We detail protocols for preparing an unzipping template, constructing and calibrating the instrument, and acquiring, processing, and analyzing unzipping data. We also summarize major results from utilization of this technique for the studies of nucleosome structure, dynamics, positioning, and remodeling.

引用

页码：29 / 58

页数：30

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