Jiulongibacter sediminis gen. nov., sp nov., a new member of the family Cytophagaceae, isolated from the surface sediment of the Jiulong River in China

A bacterial strain, designated JN14-9(T), was isolated from surface sediment of the Jiulong River and characterized in a taxonomic study using a polyphasic approach. Strain JN14-9(T) was Gram-stain-negative, non-motile, rod-shaped and orange-pigmented. It can grow at 15-37 degrees C (optimum 25-30 degrees C), at pH 7-8 (optimum pH 7) and in 2-6 % (w/v) NaCl (optimum 3-4 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain JN14-9(T) formed an independent lineage related to the family Cytophagaceae with low 16S rRNA gene sequence similarities (<92.5 %) to members of other genera with validly published names. The principal fatty acids of strain JN14-9(T) were summed feature 3 (C-16:1 omega 6C/C-16:1 omega 7C) and iso-C-15:0. The isoprenoid quinone was identified as MK-7. The major polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, three aminophospholipids and five unidentified phospholipids. The DNA G+C content was 41.6 mol%. Results of phenotypic, phylogenetic and chemotaxonomic analyses clearly indicate that strain JN14-9(T) represents a novel species of a new genus within the family Cytophagaceae, for which the name Jiulongibacter sediminis gen. nov., sp. nov. is proposed. The type strain of the type species is JN14-9(T) (=MCCC 1A00733(T)=KCTC 42153(T)).