Identifying Chromatin Readers Using a SILAC-Based Histone Peptide Pull-Down Approach

被引：11

作者：

Vermeulen, Michiel ^{[1
]}

机构：

[1] Univ Med Ctr Utrecht, NL-3584 CG Utrecht, Netherlands

来源：

NUCLESOMES, HISTONES & CHROMATIN, PT A | 2012年 / 512卷

关键词：

TO-PROLINE CONVERSION; PROTEOMICS; PROTEINS; DNA;

D O I：

10.1016/B978-0-12-391940-3.00007-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Posttranslational modifications (PTMs) on core histones regulate essential processes inside the nucleus such as transcription, replication, and DNA repair. An important function of histone PTMs is the recruitment or stabilization of chromatin-modifying proteins, which are also called chromatin "readers." We have developed a generic SILAC-based peptide pull-down approach to identify such readers for histone PTMs in an unbiased manner. In this chapter, the workflow behind this method will be presented in detail.

引用

页码：137 / 160

页数：24

共 3 条

[1] Versatile and efficient chromatin pull-down methodology based on DNA triple helix formation
Isogawa, Asako
Fuchs, Robert P.
Fujii, Shingo
SCIENTIFIC REPORTS, 2018, 8
[2] Pull-Down of Metalloproteins in Their Native States by Using Desthiobiotin-Based Probes
Ngo, Chinh
Mehta, Radhika
Aggarwal, Kanchan
Fikes, Audrey G.
Santos, Ines C.
Greer, Sylvester M.
Que, Emily L.
CHEMBIOCHEM, 2019, 20 (08) : 1003 - 1007
[3] An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes
Zhai, Guijin
Dong, Hanyang
Guo, Zhenchang
Feng, Wei
Jin, Jin
Zhang, Tao
Chen, Cong
Chen, Pu
Tian, Shanshan
Bai, Xue
Shi, Lei
Fan, Enguo
Zhang, Yukui
Zhang, Kai
ANALYTICAL CHEMISTRY, 2018, 90 (19) : 11385 - 11392

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