Selenium Exerts Protective Effects Against Fluoride-Induced Apoptosis and Oxidative Stress and Altered the Expression of Bcl-2/Caspase Family

被引：42

作者：

Gao, Jiping ^{[1
]}

Tian, Xiaolin ^{[2
,3
]}

Yan, Xiaoru ^{[1
]}

Wang, Yu ^{[1
]}

Wei, Jianing ^{[1
]}

Wang, Xiaotang ^{[1
]}

Yan, Xiaoyan ^{[2
]}

Song, Guohua ^{[1
,4
]}

机构：

[1] Shanxi Med Univ, Shanxi Key Lab Expt Anim Sci & Human Dis Anim Mod, Lab Anim Ctr, Rd Xinjian 56, Taiyuan 030001, Peoples R China

[2] Shanxi Agr Univ, Shanxi Key Lab Ecol Anim Sci & Environm Med, Taigu 030801, Peoples R China

[3] Shanxi Med Univ, Sch Publ Hlth, Taiyuan 030001, Shanxi, Peoples R China

[4] Shanxi Med Univ, Mental Hlth Hosp, St Nanshifang 55, Taiyuan 030001, Shanxi, Peoples R China

来源：

BIOLOGICAL TRACE ELEMENT RESEARCH | 2021年 / 199卷 / 02期

关键词：

Sodium fluoride; Selenium; NRK-52E cells; Oxidative stress; Apoptosis; SODIUM-FLUORIDE; PROTEIN EXPRESSION; BCL-2; PROTEINS; MITOCHONDRIA; TOXICITY; KIDNEY; CELLS; LIVER; FLUOROSIS; MECHANISM;

D O I：

10.1007/s12011-020-02185-w

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluoride is widely distributed in nature, and at high concentrations, it targets the kidney and especially proximal tubule epithelial cells. Selenium is a typical trace element beneficial to humans, and the role of selenium in the prevention and treatment of fluoride-induced organ damage is an important research topic. The purpose of this study was to investigate the possible protective effects of selenium against fluoride-induced oxidative stress and apoptosis in rat renal tubular epithelial cells. We showed that the activity of antioxidant enzymes (superoxide dismutase and glutathione peroxidase) and total antioxidant capacity were significantly reduced in NaF-treated normal rat kidney cells (NRK-52E), whereas the levels of nitrogen monoxide (NO) and malondialdehyde (MDA) were significantly increased. Moreover, the number of apoptotic cells, mRNA expression of Bax, Bad, caspase-3, caspase-8, and caspase-9, and protein expression of Bax were elevated, while mitochondrial membrane potential and the protein expression of Bcl-2 were reduced. Compared with the NaF group, pretreatment with selenium enhanced the activity of antioxidant enzymes, mitochondrial membrane potential, and protein expression of Bcl-2, while the levels of NO and MDA, number of apoptotic cells, mRNA expression of Bax, Bad, caspase-3, caspase-8, and caspase-9, and protein expression of Bax were decreased. In conclusion, selenium exerted remarkable protective effect against NaF-induced oxidative stress and apoptosis and altered the expression of Bcl-2/caspase family.

引用

页码：682 / 692

页数：11