Bioethanol production from alkaline-pretreated sugarcane bagasse by consolidated bioprocessing using Phlebia sp MG-60

被引:46
作者
Le Duy Khuong [1 ,3 ]
Kondo, Ryuichiro [2 ]
De Leon, Rizalinda [1 ]
To Kim Anh [3 ]
Shimizu, Kuniyoshi [2 ]
Kamei, Ichiro [4 ]
机构
[1] Univ Philippines Diliman, Environm Engn Programme, Dept Chem Engn, Quezon City 1011, Philippines
[2] Kyushu Univ, Fac Agr, Dept Agroenvironm Sci, Fukuoka 8128581, Japan
[3] Hanoi Univ Sci & Technol, Sch Food Technol & Biotechnol, Dept Microbiol Biochem Mol Biol, Hanoi, Vietnam
[4] Miyazaki Univ, Fac Agr, Dept Forest & Environm Sci, Miyazaki 8892192, Japan
关键词
Consolidated bioprocessing; Sugarcane bagasse; White-rot fungi; Bioethanol; Alkaline pretreatment; WHITE-ROT FUNGUS; ETHANOL-PRODUCTION; CANE BAGASSE; SIMULTANEOUS SACCHARIFICATION; LIGNOCELLULOSIC MATERIALS; HYDROLYSIS; FERMENTATION; BIOMASS; ACID; BIOCONVERSION;
D O I
10.1016/j.ibiod.2013.12.008
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Optimization of alkaline pretreatment of sugarcane bagasse for consolidated bioprocessing fermentation by the cellulose-fermenting fungus Phlebia sp. MG-60 was studied. The lignin and xylan contents of bagasse were decreased and ethanol production from each pretreated sugarcane bagasse by MG-60 was increased in an alkaline concentration-dependent manner. The fungus produced cellulase and xylanase rapidly over 120 h. When this fungus was cultured with 20 g L-1 of sugarcane bagasse pretreated with NaOH (0.8 wt%, 121 degrees C, 60 mm), 4.5 g L-1 ethanol was produced, equivalent to 210 mg ethanol per gram of the original untreated bagasse after 240 h fermentation, giving ethanol yields of 65.7% of the theoretical maximum. These data suggest that Phlebia sp. MG-60 is a potential candidate for ethanol production from alkali-pretreated bagasse in a single bioreactor, without enzymatic or chemical hydrolysis. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:62 / 68
页数:7
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