CD151 regulates HGF-stimulated morphogenesis of human breast cancer cells

被引：32
作者：
Klosek, Sebastian K. [1 ]
Nakashiro, Koh-ichi [1 ]
Hara, Shingo [1 ]
Goda, Hiroyuki [1 ]
Hasegawa, Hitoshi [2 ]
Hamakawa, Hiroyuki [1 ]
机构：
[1] Ehime Univ, Dept Oral & Maxillofacial Surg, Grad Sch Med, Toon, Ehime 7910295, Japan
[2] Ehime Univ, Dept Bioregulatory Med, Grad Sch Med, Toon, Ehime 7910295, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 379卷 / 04期
关键词：
CD; 151; HGF; c-Met; Integrin alpha 3/alpha 6; Cellular morphogenesis; Breast cancer; HEPATOCYTE GROWTH-FACTOR; TETRASPANIN CD151; MOLECULAR-CLONING; INTEGRIN; ADHESION; EXPRESSION; COMPLEX; PALMITOYLATION; ALPHA-3-BETA-1; ALPHA-6-BETA-4;
D O I：
10.1016/j.bbrc.2009.01.023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We previously demonstrated that CD151 forms a functional complex with c-Met and integrin alpha 3/alpha 6 in human salivary gland cancer cells. In the current study, we investigated the involvement of CD151, c-Met, and integrin alpha 3/alpha 6 in the cellular morphogenesis of human breast cancer cells, Knockdown of CD151, integrin alpha 3, or integrin alpha 6 expression abolished branching morphogenesis. Decreased c-Met expression in these cells led to the formation of rudimentary networks and prevented their conversion. Furthermore, hepatocyte growth factor (HGF) promoted cellular morphogenesis by accelerating network reorganization. Immunoprecipitation revealed a specific association between CD151 and c-Met, The involvement of CD151 and integrin alpha 3/alpha 6 in HGF-depenclent signaling was confirmed by the decreased Akt phosphorylation in cells lacking CD151, integrin alpha 3, or integrin alpha 6. Hence, the regulation of CD151 expression might contribute to changes in HGF/c-Met signaling and thereby modulate the phenotypic characteristics of cancer cells. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：1097 / 1100
页数：4
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