A fast response fluorescence probe specific for hypochlorous acid detection and its applications in bioimaging

被引：28

作者：

Jia, Hongmin ^{[1
]}

Xia, Shuhe ^{[1
]}

Feng, Huan ^{[1
]}

Meng, Qingtao ^{[1
]}

Duan, Chengchen ^{[2
]}

Zhang, Zhiqiang ^{[1
]}

Zhang, Run ^{[2
]}

机构：

[1] Univ Sci & Technol Liaoning, Sch Chem Engn, Anshan 114051, Liaoning, Peoples R China

[2] Univ Queensland, Australian Inst Bioengn & Nanotechnol, Brisbane, Qld 4072, Australia

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2018年 / 16卷 / 12期

基金：

澳大利亚研究理事会; 英国医学研究理事会; 中国国家自然科学基金;

关键词：

REAL WATER SAMPLES; LIVING CELLS; ENDOGENOUS HYPOCHLORITE; LIVE CELLS; IN-VIVO; SENSITIVE DETECTION; COMPLEX SYNTHESIS; FLOW-CYTOMETRY; TAP WATER; MITOCHONDRIA;

D O I：

10.1039/c8ob00036k

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Quantitative detection and visualization of hypochlorous acid (HOCl) in biological samples has emerged as a hot topic in biochemical research because of the important role of this biomolecule in live organisms. In this contribution, a novel 1,8-naphthalimide-based fluorescence probe, DNPH-NA, was designed and synthesized for HOCl detection in aqueous solutions and in biological systems. In the presence of HOCl, significant changes in absorption and fluorescence spectra were observed, which allow for the quantitative detection of HOCl in PBS buffer. The detection limit for HOCl was determined to be 50 nM. In the presence of HOCl, the fluorescence response of DNPH-NA was found to be completed within 2 seconds. The desirable features of DNPH-NA for the detection of HOCl in aqueous solutions, such as its high sensitivity and selectivity, reliability at physiological pH, rapid fluorescence response, and biocompatibility enabled its application in the detection of HOCl in biological samples. Imaging of exogenous HOCl in live HeLa cells and endogenous HOCl generation in live J774A.1 macrophage cells and zebrafish was then successfully performed using DNPH-NA as a fluorescence probe.

引用

页码：2074 / 2082

页数：9

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