Gold Nanowire Based Electrical DNA Detection Using Rolling Circle Amplification

被引:93
作者
Russell, Camilla [1 ]
Welch, Ken [3 ]
Jarvius, Jonas [4 ]
Cai, Yixiao [3 ]
Brucas, Rimantas [3 ]
Nikolajeff, Fredrik [3 ]
Svedlindh, Peter [3 ]
Nilsson, Mats [1 ,2 ]
机构
[1] Uppsala Univ, Dept Immunol Genet & Pathol, SE-75185 Uppsala, Sweden
[2] Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, S-11418 Stockholm, Sweden
[3] Uppsala Univ, Dept Engn Sci, SE-75121 Uppsala, Sweden
[4] Q Linea AB, SE-75237 Uppsala, Sweden
基金
瑞典研究理事会;
关键词
gold nanoparticles; rolling circle amplification; nanoelectronics; gold conjugation; metal enhancement; PADLOCK PROBES; METALLIZATION; ALIGNMENT; PRECISE; ACID;
D O I
10.1021/nn4058825
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We present an electrical sensor that uses rolling circle amplification (RCA) of DNA to stretch across the gap between two electrodes, interact with metal nanoparticle seeds to generate an electrically conductive nanowire, and produce electrical signals upon detection of specific target DNA sequences. RCA is a highly specific molecular detection mechanism based on DNA probe circularization. With this technique, long single-stranded DNA with simple repetitive sequences are produced. Here we show that stretched RCA products can be metalized using silver or gold solutions to form metal wires. Upon metallization, the resistance drops from T Omega to k Omega for silver and to Omega for gold. Metallization is seeded by gold nanoparticles aligned along the single-stranded DNA product through hybridization of functionalized oligonucleotides. We show that combining RCA with electrical DNA detection produces results in readout with very high signal-to-noise ratio, an essential feature for sensitive and specific detection assays. Finally, we demonstrate detection of 10 ng of Escherichia coli genomic DNA using the sensor concept.
引用
收藏
页码:1147 / 1153
页数:7
相关论文
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