Resistance training and redox homeostasis: Correlation with age associated genomic changes

被引:55
作者
Dimauro, Ivan [1 ]
Scalabrin, Mattia [1 ]
Fantini, Cristina [1 ]
Grazioli, Elisa [1 ]
Valls, Maria Reyes Beltran [1 ]
Mercatelli, Neri [1 ]
Parisi, Attilio [2 ]
Sabatini, Stefania [1 ]
Di Luigi, Luigi [3 ]
Caporossi, Daniela [1 ]
机构
[1] Univ Rome Foro Italico, Unit Biol Genet & Biochem, Dept Movement Human & Hlth Sci, Piazza Lauro de Bosis 15, I-00135 Rome, Italy
[2] Univ Rome Foro Italico, Unit Sport Med, Dept Movement Human & Hlth Sci, Rome, Italy
[3] Univ Rome Foro Italico, Unit Endocrinol, Dept Movement Human & Hlth Sci, Rome, Italy
关键词
Telomeres; DNA methylation; Myeloperoxidase; TrxR1; MnSOD; LEUKOCYTE TELOMERE LENGTH; MESSENGER-RNA EXPRESSION; PHYSICAL-ACTIVITY; OXIDATIVE STRESS; GENE-EXPRESSION; DNA METHYLATION; EXERCISE; DECLINE; BIOMARKER; CELLS;
D O I
10.1016/j.redox.2016.09.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Regular physical activity is effective as prevention and treatment for different chronic conditions related to the ageing processes. In fact, a sedentary lifestyle has been linked to a worsening of cellular ageing biomarkers such as telomere length (TL) and/or specific epigenetic changes (e.g. DNA methylation), with increase of the propensity to aging-related diseases and premature death. Extending our previous findings, we aimed to test the hypothesis that 12 weeks of low frequency, moderate intensity, explosive-type resistance training (EMRT) may attenuate age-associated genomic changes. To this aim, TL, global DNA methylation, TRF2, Ku80, SIRTI, SIRT2 and global protein acetylation, as well as other proteins involved in apoptotic pathway (Bcl-2, Bax and Caspase-3), antioxidant response (TrxR1 and MnSOD) and oxidative damage (myeloperoxidase) were evaluated before and after EMRT in whole blood or peripheral mononuclear cells (PBMCs) of elderly subjects. Our findings confirm the potential of EMRT to induce an adaptive change in the antioxidant protein systems at systemic level and suggest a putative role of resistance training in the reduction of global DNA methylation. Moreover, we observed that EMRT counteracts the telomeres' shortening in a manner that proved to be directly correlated with the amelioration of redox homeostasis and efficacy of training regime, evaluated as improvement of both muscle's power/strength and functional parameters. (C) 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.
引用
收藏
页码:34 / 44
页数:11
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