New oligosaccharides prepared by acid hydrolysis of the polysaccharides from Nerium indicum Mill and their anti-angiogenesis activities

被引：25

作者：

Hu, Ke ^{[1
,2
]}

Liu, Qin ^{[2
]}

Wang, Shunchun ^{[1
]}

Ding, Kan ^{[2
]}

机构：

[1] Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China

来源：

CARBOHYDRATE RESEARCH | 2009年 / 344卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Nerium indicum; Galactooligosaccharide; Anti-angiogenesis activity;

D O I：

10.1016/j.carres.2008.10.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To discover drug candidates with anti-angiogenesis activity for cancer therapeutics, three galactooligosaccharides (OJ1-OJ3) were prepared by acid hydrolysis of the polysaccharides from Nerium indicum Mill. Their structures were characterized using sugar analysis, methylation analysis, and both 1D and 2D NMR spectroscopy, complemented by mass spectrometry. They were hexasaccharide (0J1), a pentasaccharide (OJ2), and an undecasaccharide (OJ3), which was a new linear galactan. Bioactivity testing in vitro showed that OJ2 and OJ3 significantly inhibited the HMEC-1 (human microvascular endothelial cell) cell tube formation. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：198 / 203

页数：6

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